水产科技情报2024，Vol.51Issue(1)：44-51,8.DOI:10.16446/j.fsti.20220300122
氨氮胁迫对翘嘴鳜幼鱼抗氧化酶、消化酶活性及应激相关基因表达的影响
Effects of acute ammonia nitrogen stress on the activities of antioxidant enzyme and digestive enzyme and the expression pattern of stress-related genes in juvenile mandarin fish(Siniperca chuatsi)
摘要
Abstract
In order to understand the response mechanism of antioxidant system and digestion system of mandarin fish(Siniperca chuatsi)under ammonia nitrogen stress,the activities of related enzymes[glutathione(GSH),superoxide dismutase(SOD),catalase(CAT),lipid peroxide(LPO),pepsin,gastric amylase(AMS),gastric lipase(LPS)]and the expression of stress-related genes(IL-1β,IL-8,TNF-α,HSP90α)in the liver and stomach of juvenile S.chuatsi with body weight of(15.27±0.67)g under six ammonia nitrogen stress concentrations(0,10,20,30,40,50 mg/L)were studied.The results showed that the activities of SOD,CAT and GSH in the liver increased at first and then decreased,while LPO activities increased gradually under 96 h of ammonia nitrogen stress.The gene express of IL-1β and HSP90α showed a decreasing trend after increasing,while TNF-α express increased gradually,and IL-8 express showed the trend of decreasing,increasing and decreasing.The activities of pepsin increased first and then decreased,while AMS and LPS activities increased gradually.Studies have shown that fish can respond to oxidative stress damage by increasing the activities of antioxidant enzymes and up-regulation of stress-related gene expression,and at the same time induce the increase of digestive enzyme activities in the stomach to provide energy for the body under the stress of low concentration of ammonia nitrogen.However,the activities of antioxidant enzymes and the expression of HSP90α gene were inhibited by high concentration ammonia nitrogen stress.关键词
氨氮胁迫/翘嘴鳜/抗氧化酶/消化酶/炎症相关基因/酶活力Key words
acute ammonia nitrogen stress/mandarin fish/antioxidant enzyme/digestive enzyme/gene related to inflammation/enzyme activity引用本文复制引用
孙阿君,丁炜东,曹丽萍,曹哲明,邴旭文..氨氮胁迫对翘嘴鳜幼鱼抗氧化酶、消化酶活性及应激相关基因表达的影响[J].水产科技情报,2024,51(1):44-51,8.基金项目
江苏省种业振兴揭榜挂帅项目(JBGS[2021]034) （JBGS[2021]034）
中国水产科学研究院淡水渔业研究中心基本科研业务费项目(2023JBFM10、2019JBFZ01) （2023JBFM10、2019JBFZ01）
江苏省农业自主创新项目[cx(17)3005]. （17）
