水产学报2024，Vol.48Issue(1)：40-51,12.DOI:10.11964/jfc.20210913087
低温胁迫对暗纹东方鲀肠道氧化应激、细胞凋亡及肠道微生物组成的影响
Effects of low temperature stress on the oxidative stress,apoptosis and intestinal microbial composition in intestine of Takifugu fasciatus
摘要
Abstract
In order to explore the effects of low temperature stress on intestinal oxidative stress,apoptosis and intestinal microbial composition of Takifugu fasciatus,experiments were conducted to measure oxidative stress,apoptosis and intestinal microbe-related indicators at 3 treatment temperatures(25,19 and 13℃)and 4 sampling time periods(0,6,24 and 96 h).Compared with the control group,the results showed that as the temperature decreased,intestinal villi underwent necrosis,hyperplasia and enlargement of goblet cells,the enzymatic activities of total superoxide dismutase(T-SOD),catalase(CAT),glutathione peroxidase(GSH-Px)and the content of malondialdehyde(MDA)increased significantly,while the diversity of gut microbial composition decreased.Changes in gut microbial abundance,such as Xanthobacter,Paracocuus,Lactococcus,and Bididobacter increased with decreasing temperature,Clostridium,Aeromonas and Geobacillus decreased with increasing temperature,and the expression of apoptosis-related genes(caspase3,caspase7,caspase9,p53,Bax,Bcl-2)increased with the decrease of temperature.In addition,we found that the alterations in the abundance of Paracocuus and Geobacil-lus had Pearson correlations with intestinal oxidative stress and apoptosis,which may be potential microbes to reflect whether the T.fasciatus is in a state of oxidative stress and apoptosis.The results of this study provide refer-ence for the healthy breeding of T.fasciatus.关键词
暗纹东方鲀/低温/肠道微生物/氧化应激/细胞凋亡Key words
Takifugu fasciatus/low temperature/intestinal microbes/oxidative stress/apoptosis分类
农业科技引用本文复制引用
付东勇,张艺然,褚鹏,王浩哲,张凯,毕宜慧,孙亦如,王涛,尹绍武..低温胁迫对暗纹东方鲀肠道氧化应激、细胞凋亡及肠道微生物组成的影响[J].水产学报,2024,48(1):40-51,12.基金项目
江苏省种业振兴"揭榜挂帅"项目[JBGS(2021)034] （2021）
国家自然科学基金(32172948,31800436) （32172948,31800436）
江苏省农业科技自主创新项目[CX(22)2029] （22）
国家重点研发计划"蓝色粮仓科技创新"(2018YFD0900301) （2018YFD0900301）
江苏省自然科学基金(BK20180728,BK20210563) the"JBGS"Project of Seed Industry Revitalization in Jiangsu Province[JBGS(2021)034] （BK20180728,BK20210563）
National Natural Science Foundation of China(32172948,31800436) （32172948,31800436）
Jiangsu Agriculture Science and Techno-logy Innovation Fund[CX(22)2029] （22）
National Key Research and Development Program"Blue Granary Techno-logy Innovation"(2018YFD0900301) （2018YFD0900301）
Natural Science Foundation of Jiangsu Province(BK20180728,BK20210563) （BK20180728,BK20210563）
