水产学报2024，Vol.48Issue(1)：82-92,11.DOI:10.11964/jfc.20210612894
基于线粒体COⅠ基因序列的梭鲈野生群体遗传结构
Genetic structure of wild Sander lucioperca populations based on COⅠ gene
摘要
Abstract
The pike-perch(Sander lucioperca)is a native fish species in European and Asian river basins.Owing to its fast growth rate,strong adaptability,and high nutritional value,it has become a promising candidate species for aquaculture.In China,artificial reproduction of pike-perch has been realized,and most of its parents are caught from wild populations.However,the genetic background and diversity information of wild populations are still limited,which is not conducive to the establishment of genetic breeding management strategies.In order to invest-igate the genetic structure of S.lucioperca wild populations,we analyzed the genetic differences of six popula-tions in China and two populations in Central Asia using partial mitochondrial COⅠgene sequences,and com-pared them with the haplotype sequences of European populations.As a result,5 variable sites were detected in the 640 bp COⅠgene sequence,and 7 haplotypes were defined,among which Hap1 was the shared haplotype between eight populations,and was identical to HapA of European groups.The proportion of Hap1 in Chinese populations(93.36%)was higher than that in Central Asian populations(72.58%)and European population(55.56%).Hap2 and Hap3 were specific haplotypes of Chinese populations,while Hap4,Hap5,Hap6,and Hap7 were specific haplotypes of Central Asian populations.The cluster and network analysis of haplotype sequences showed that Hap1/A was the ancestral haplotype in Sander lucioperca populations.The specific haplotype of Chinese and Central Asian populations belonged to Hap1/A subtype with only 1-2 loci variation compared with the ancestral haplotype,which was quite different from the specific haplotype of European populations.The number of haplotypes per population ranged from one to four,with the largest number of haplotypes in the Zaysan Lake(ZS)population,while there was only one haplotype(Hap1)in Tengger Lake(NX),Xingkai Lake(XK)and Yalu River(YJ)populations in China.The haplotype diversity(Hd)and nucleotide diversity(π)of TS population were the highest(Hd=0.514±0.069;Pi=0.000 80±0.000 11),followed by ZS population,while the diversity parameters of six populations in China were lower.The AMOVA analysis showed that the genetic variation among S.lucio-perca populations accounted for 20.74%,and the degree of genetic differentiation(Fst)among populations was rel-atively high(0.15≤Fst=0.207 36<0.25).The genetic differentiation of the TS and the ZS,the TS and the six Chinese populations all were great(Fst>0.25).Among Chinese populations,Heihe(HH)population had great genetic differentiation against other populations,while there was no genetic differentiation between the other five populations.The phylogenetic tree based on genetic distance showed that six populations from China and ZS pop-ulation from Kazakhstan were clustered into one branch,while TS population from Uzbekistan was a separate branch.The results of this study provide a reference for the breeding and releasing management of S.lucioperca populations.关键词
梭鲈/线粒体COⅠ基因/野生群体/遗传结构Key words
Sander lucioperca/mitochondrial cytochrome c oxidase subunitⅠ(COⅠ)gene/wild population/genetic structure分类
生物科学引用本文复制引用
鲁翠云,孙志鹏,曹顶臣,耿龙武,那荣滨,吴学工,郑先虎..基于线粒体COⅠ基因序列的梭鲈野生群体遗传结构[J].水产学报,2024,48(1):82-92,11.基金项目
国家重点研发计划(2019YFD0900405) （2019YFD0900405）
农业农村部财政专项"西北地区重点水域渔业资源与环境调查" （）
中央级公益性科研院所基本业务费专项(2020TD56,HSY202009Q) （2020TD56,HSY202009Q）
国家水产种质资源平台(2020DKA30470) National Key R&D Program of China(2019YFD0900405) （2020DKA30470）
Ministry of Agriculture Finance Special Project"Investigation on Fishery Resources and Environment in Key Waters of Northwest China" （）
Cent-ral Public-interest Scientific Institution Basal Research Fund,CAFS(2020TD56,HSY202009Q) （2020TD56,HSY202009Q）
National Infra-structure of Fishery Germplasm Resource(2020DKA30470) （2020DKA30470）
