水产学报2024，Vol.48Issue(1)：164-174,11.DOI:10.11964/jfc.20211013103
响应面法优化长茎葡萄蕨藻多糖提取工艺及抗氧化活性
Response surface methodology-based optimization of extraction process of polysaccharides from Caulerpa lentillifera and their antioxidant activity
摘要
Abstract
Caulerpa lentillifera is a kind of perennial large edible seaweed,which is also known as"sea grape"because of its upright stem globule glittering and translucent,similar in appearance to grapes.C.lentillifera is also one of the most suitable seaweeds for human consumption.It is rich in nutrients and contains a variety of amino acids,vitamins,polysaccharides and fatty acids needed by the human body.Among them,polysaccharide is the most important active component in C.lentillifera.According to research,the crude polysaccharide content of C.lentillifera accounts for 34.00%-64.97%of its dry weight,which is higher than that of most edible algae,such as Scagassum,Hizikia fusifarme and Undaria pinnatifida.At present,the main extraction methods for polysacchar-ides of C.lentillifera are water extraction,alkali extraction and ultrasonic-assisted extraction,and the extraction rate is 3.22%-11.30%,but there are still a large amount of polysaccharides that remains un-extracted.In order to develop a mild,efficient and low-cost extraction process of polysaccharides from C.lentillifera,and to explore the antioxidant activity of the polysaccharide,we screened five existing extraction methods of polysaccharides from C.lentillifera.Through single factor experiment and response surface experiment,the ratio of material to solution,extraction temperature,extraction time,amount of papain and extraction times were optimized,and the optimum process parameters were obtained.Polysaccharide concentration was determined by anthrone sulfate method in the experiment.In addition,we investigated the scavenging activity of the polysaccharide on DPPH and ABTS free radicals.The results showed that adding papain is the most efficient method to extract polysaccharides from C.len-tillifera.The order of the factors affecting the extraction rate of polysaccharide from C.lentillifera is as follows:ratio of material to solution>extraction temperature>extraction time,and the ratio of material to solution showed an extremely significant influence on the extraction rate of the polysaccharide(P<0.001).The best extraction para-meters after optimization were as follows:ratio of material to solution is 1∶45,extraction temperature 60℃,extraction time 3 h,extraction count 2 times,the addition amount of papain 2.0%.Under this condition,the extrac-tion rate of polysaccharides can reach 41.24%±0.09%.In addition,polysaccharides from C.lentillifera had good scavenging activities on DPPH and ABTS free radicals,and their IC50 values were 2.32 and 0.67 mg/mL,respect-ively.This study shows that the optimized papain enzymatic hydrolysis can improve the extraction rate of polysac-charides from C.lentillifera effectively,among which the ratio of material solution is the most important factor affecting this method.Besids,the polysaccharides of C.lentilliferahave good antioxidant activity.关键词
长茎葡萄蕨藻/多糖/木瓜蛋白酶/响应面法/抗氧化性Key words
Caulerpa lentillifera/polysaccharide/papain/response surface method/antioxidant resistance分类
农业科技引用本文复制引用
童艳梅,潘传燕,曾地刚,赵永贞,陈秀荔,胡庭俊,刘青云,李强勇,冯鹏霏,杨春玲,彭敏,朱威霖..响应面法优化长茎葡萄蕨藻多糖提取工艺及抗氧化活性[J].水产学报,2024,48(1):164-174,11.基金项目
广西科技计划项目"南美白对虾与长茎葡萄蕨藻工厂化生态共养模式研究"(桂科AB18221113) （桂科AB18221113）
国家虾产业技术体系建设专项(CARS-48) Guangxi Science and Technology Plan Project"Research on Industrial Ecological Co-Culture Model of Litopenaeus vannamei and Caulerpa lentillifera"(GK AB18221113) （CARS-48）
National Shrimp Industry Techno-logy System Construction Project(CARS-48) （CARS-48）
