山东农业科学2023，Vol.55Issue(12)：1-8,8.DOI:10.14083/j.issn.1001-4942.2023.12.001
玉米ZmWRKY36基因的生物信息学及功能分析
Bioinformatics and Functional Analysis of ZmWRKY36 Gene in Maize
摘要
Abstract
As one of the largest families of transcription factors in the plant,the WRKY proteins play crucial roles in plant growth and development,defense regulation and stress response.In this research,we cloned a maize WRKY transcription factor gene ZmWRKY36,and conducted the bioinformatics and functional analysis.The results showed that the open reading frame of ZmWRKY36 gene was 1 002 bp,and it encoded 333 amino acids.The molecular weight of ZmWRKY36 was 35.8 kDa,and its isoelectric point was 5.66,so it belonged to an acidic protein;its instability coefficient was 67.16,indicating that it was a highly unstable pro-tein;its hydrophilicity index was-0.526,indicating that it was a hydrophilic protein;it contained one WRKY domain and one C2 HC zinc-finger motif,so it was a member of the subgroupⅢfamily of WRKY transcription factor;it showed close relationships with SbWRKY64,PmWRKY4 and SvWRKY4.Further analysis results showed that ZmWRKY36 protein was localized in the nucleus and had strong transcriptional activation activity.The results of qRT-PCR indicated that the expression of ZmWRKY36 gene was tissue-specific with higher level in root and leaves,and was significantly induced by drought stress and salt stress,which suggested that the transcription factor ZmWRKY36 might play a key role in the process of maize resisting environmental drought and salt stresses.关键词
玉米/ZmWRKY36/生物信息学分析/功能分析/干旱胁迫/盐胁迫Key words
Maize/ZmWRKY36/Bioinformatics analysis/Functional analysis/Drought stress/Salt stress分类
农业科学引用本文复制引用
王文丽,张恩盈,穆春华,张发军,刘霞,冷冰莹,姚国旗,闫振伟,刘巍,王昀..玉米ZmWRKY36基因的生物信息学及功能分析[J].山东农业科学,2023,55(12):1-8,8.基金项目
山东省重点研发计划项目"玉米抗锈病机制及绿色防控体系集成与应用"(2022CXGC010607) （2022CXGC010607）
国家自然科学基金青年基金项目"细胞分裂素调控植物气孔发育分子机理的研究"(31900249) （31900249）
山东省农业科学院农业科技创新工程项目(CXGC2022E02) （CXGC2022E02）
