山东农业科学2023，Vol.55Issue(12)：9-16,8.DOI:10.14083/j.issn.1001-4942.2023.12.002
枣PAO基因的鉴定及在果实发育和贮藏过程中的表达分析
Identification and Expression Analysis of PAO Gene during Fruit Development and Storage of Ziziphus jujuba
摘要
Abstract
Polyamine oxidase(PAO)is a flavin adenine dinucleotide dependent enzyme that catalyzes the catabolism of polyamines(PA)and plays a crucial role in plant growth,development and stress response.The genome-wide screening of PAO genes in jujube was conducted,and three ZjPAO genes(ZjPAO1,ZjPAO2,ZjPAO3)were identified,then their conserved domains,gene structures,chromosomal positions,evolutionary relationships,subcellular localization and promoter cis acting elements were analyzed.The results showed that the three ZjPAO genes were distributed on Chr4,Chr5 and Chr8,respectively,with relatively conservative structural characteristics and the Amino-oxidase domain.By constructing the phylogenetic trees of the PAO genes of Arabidopsis,tomato,peach and jujube,we found that they could be divided into four catego-ries,and the ZjPAO geneswere closely related to the PAO genes in peach.The promoter of the ZjPAO genes contained various cis acting elements related with such as hormones and stress responses,which were predicted to play roles in jujube growth and development.In addition,the transcriptome data analysis showed that both ZjPAO1 and ZjPAO2 expressed at different stages of jujube fruit development.Under exogenous salicylic acid treatment,the ZjPAO1 gene was significantly induced to express.The results of this study could contribute to understanding the mechanisms of delaying ripening and aging of fresh jujube fruits.关键词
枣/多胺氧化酶(PAO)/基因鉴定/生物信息学分析/基因表达/果实发育/采后贮藏Key words
Ziziphus jujuba/Polyamine oxidase(PAO)/Gene identification/Bioinformatics analysis/Gene expression/Fruit growth and development/Post-harvest storage分类
农业科技引用本文复制引用
李丽莉,樊丁宇,杨磊,靳娟,郝庆..枣PAO基因的鉴定及在果实发育和贮藏过程中的表达分析[J].山东农业科学,2023,55(12):9-16,8.基金项目
国家重点研发计划项目(2021YFD1000404) （2021YFD1000404）
现代农业产业技术体系专项(CARS-30) （CARS-30）
中央引导地方科技发展专项"特色果树种质创新与育种能力提升" （）
新疆红枣产业技术体系专项(XJCYTX-01) （XJCYTX-01）
