山东农业科学2023，Vol.55Issue(12)：17-25,9.DOI:10.14083/j.issn.1001-4942.2023.12.003
脂氧合酶基因LOX6克隆及其表达与鲜切芋头褐变的相关性
Cloning of Taro Lipoxygenase Gene LOX6 and Correlation of Its Expression with Browning of Fresh-Cut Taros
摘要
Abstract
Membrane lipid peroxidation is a primary cause of browning in fresh-cut fruits and vegetables.Lipoxygenase(LOX)plays a pivotal role in membrane lipid peroxidation.This study aimed to investigate the correlation between the LOX gene LOX6 expression and the browning of fresh-cut taros.The complete cDNA sequence of the LOX6 gene was cloned using taro cDNA as a template,and its expression pattern during the browning process was analyzed.The coding sequence(CDS)of taro LOX6 was 2 751 bp,encoding 916 amino acid residues.The similarity of animo acid sequence between LOX6 proteins in different plants was not very high,and the closest genetic relationship was observed between taro LOX6 and ZaLOX6.As the browning of fresh-cut taro intensifying,the LOX6 expression gradually increased,and showed a significant positive correla-tion with the browning indicator△E(R2=0.72);treating with exogenous vanillic acid(VA)significantly in-hibited the fresh-cut taro browning and downregulated the LOX6 gene expression.It could be concluded that the expression of LOX6 had obvious correlation with browning of fresh-cut taros,so inhibiting its expression might inhibit the browning of fresh-cut taros.The LOX6 gene promoter contained many stress response-related cis elements,suggesting that the induced expression of LOX6 by fresh-cut treatment might be a crucial factor facilitating the browning of taros after cutting.The results of this study could offer important genetic resources for cultivating browning-resistant taro,and contribute to the optimization of browning prevention and control technology for fresh-cut taros.关键词
鲜切芋头/褐变/脂氧合酶基因LOX6/低温贮藏/膜脂过氧化Key words
Fresh-cut taros/Browning/Lipoxygenase gene LOX6/Low temperature storage/Membrane lipid peroxidation分类
园艺学与植物营养学引用本文复制引用
袁晓,杨盼迪,林冲,朱云娜,王光,王斌..脂氧合酶基因LOX6克隆及其表达与鲜切芋头褐变的相关性[J].山东农业科学,2023,55(12):17-25,9.基金项目
广东省粤北食药资源利用与保护重点实验室开放基金资助项目(FMR2022001M) （FMR2022001M）
广东大学生科技创新培育专项资金(大学生科技创新培育)资助项目(pdjh2023a0470) （大学生科技创新培育）
