山东农业科学2023，Vol.55Issue(12)：26-33,8.DOI:10.14083/j.issn.1001-4942.2023.12.004
基于SSR标记的石榴品种遗传多样性分析和指纹图谱构建
Genetic Diversity Analysis and DNA Fingerprint Construction of Pomegranate Varieties Based on SSR Molecular Markers
摘要
Abstract
In order to identify the genetic diversity of major pomegranate varieties and construct their DNA fingerprints,30 pomegranate varieties were used as materials,the agarose and capillary electrophoresis were used to screen polymorphic primers,and the SSR molecular marker identification system were construc-ted.Then the DNA fingerprints of the pomegranate varieties were constructed by using the tandem codes con-taining sample,primer and allele information.The results showed that ten pairs of primers with clear and sta-ble bands and high polymorphism were selected from 36 pairs of primers.Using them,48 alleles were detected with the mean of 4.8 per every pair of primer.The average observed heterozygosity and expected heterozygosity were 0.50 and 0.60,respectively.The Shannon's index and Nei's diversity index were 1.11 and 0.59,re-spectively.The polymorphism information content ranged from 0.33 to 0.72,with the mean of 0.53.The cluster analysis results showed that the 30 varieties could be classified into 3 groups,among which,the group A in-cluded 16 varieties,the group B included 8 varieties,and the group C included 6 varieties.Based on the in-formation of samples,primers and alleles,the specific fingerprint codes and their two-dimensional codes of the 30 varieties were constructed.The results of this study could provide theoretical bases for molecular identifica-tion,development and utilization of germplasm resources of pomegranate.关键词
石榴品种/SSR分子标记/遗传多样性/DNA指纹图谱Key words
Pomegranate(Punica granatum)variety/SSR molecular marker/Genetic diversity/DNA fingerprint分类
农业科技引用本文复制引用
冯立娟,程云,王传增,焦其庆,尹燕雷..基于SSR标记的石榴品种遗传多样性分析和指纹图谱构建[J].山东农业科学,2023,55(12):26-33,8.基金项目
山东省农业科学院农业科技创新工程项目(CXGC2023A12) （CXGC2023A12）
山东省重点研发计划项目(2021LZGC007) （2021LZGC007）
枣庄学院山东省石榴精深加工工程技术研究中心/山东省石榴资源综合开发工程实验室开放课题(SLKF2021001) （SLKF2021001）
山东省农业科学院揭榜科技难题项目(SHJB2022-42) （SHJB2022-42）
