山东农业科学2023，Vol.55Issue(12)：56-62,7.DOI:10.14083/j.issn.1001-4942.2023.12.008
茶白星病侵染对茶树特征性生化成分的影响及相关性分析
Effect of Tea White Scab Disease Infection on Characteristic Biochemical Components of Tea Plant and Their Correlation Analysis
摘要
Abstract
This research aimed to investigate the effects of tea white scab disease infection on characteris-tic biochemical components in tea plant,and analyze the correlation between the components and injury extent.UV spectrophotometry and HPLC were used to determine the content of tea polyphenols,free amino acids,gallic acid,theobromine,caffeine,L-theanine,epigallocatechin(EGC),catechin(C),epicatechin(EC),epigallocatechin gallate(EGCG),(-)-gallocatechin gallate(GCG)and epigallocatechin gallate(ECG)in healthy tea leaves and the tea leaves at different damage levels caused by tea white scab disease.Furthermore,correlation analysis and path analysis were used to ascertain the relationship between the characteristic bio-chemical components and the hazard severity of tea white scab disease.The results indicated that the content of free amino acid and L-theanine in tea leaves increased by 12%to 54%and 42%to 83%respectively after the damage of tea white scab disease,while that of caffeine decreased by about 10%.From healthy fresh leaves to severely damaged leaves,the changes of theobromine,gallic acid,tea polyphenols,total catechins,ester cat-echins,EGCG,ECG and GCG contents were consistent with a tendency from decline to rise and then decline,and the highest contents were found in moderately damaged leaves.On the contrary,the changes of non-ester catechins,EGC and EC contents showed a tendency from rise to decline and then rise,and the lowest contents were also found in moderately damaged leaves.The correlation analysis showed that the contents of gallic acid and C were significantly and extremely significantly positively correlated with the disease index of tea white scab disease with the correlation coefficients as 0.680 and 0.831,respectively.The contents of free amino acids and L-theanine were extremely significantly negatively correlated with the disease index of tea white scab disease with the correlation coefficients as-0.990 and-0.913,respectively.The results of path analysis showed that L-theanine and gallic acid were the crucial biochemical factors affecting the hazard severity of tea white scab disease.关键词
茶白星病/生化成分/没食子酸/茶氨酸/相关性分析Key words
Tea white scab disease/Biochemical components/Gallic acid/L-Theanine/Correlation a-nalysis分类
农业科技引用本文复制引用
杨春,李帅,孟泽洪,张小琴,梁思慧,李燕,席亚楠,沈强..茶白星病侵染对茶树特征性生化成分的影响及相关性分析[J].山东农业科学,2023,55(12):56-62,7.基金项目
科技部国家重点研发计划"绿色宜居村镇技术创新"重点专项(2021YFD1100305) （2021YFD1100305）
贵州省科技计划项目(黔科合平台人才[2020]2103) （黔科合平台人才[2020]2103）
