上海农业学报2023，Vol.39Issue(6)：12-16,5.DOI:10.15955/j.issn1000-3924.2023.06.03
颗粒野生稻愈伤的诱导继代与超低温保存
Callus induction,subculture and cryopreservation of Oryza granulata
摘要
Abstract
To preserve the resources of Oryza granulata,two kinds of O.granulata introduced from the International Rice Research Institute were selected to induce callus and establish tissue culture rapid propagation system,and the callus tissue was preserved at ultra-low temperature.The results showed that the calli of wild rice could obtained by seed induction and radicle induction after germination.The alternative subculture of NB and MS medium was helpful to improve the callus activity.By the combination of high sugar pre-culture and drying treatment,the calli of two kinds of O.granulata survived after cryopreservation,with callus proliferation rates of 34.24％and 23.95％,and the regenerated calli could differentiate into new plants.The combination of tissue culture and rapid propagation technology with cryopreservation provided an effective way for the preservation of O.granulata germplasm.关键词
颗粒野生稻/愈伤/超低温保存Key words
Oryza granulata/Callus/Cryopreservation分类
农业科技引用本文复制引用
韩静,林田,周丽,滕小英,龙萍,李天菲..颗粒野生稻愈伤的诱导继代与超低温保存[J].上海农业学报,2023,39(6):12-16,5.基金项目
上海市科委项目(21DZ2290600) （21DZ2290600）
