上海农业学报2023，Vol.39Issue(6)：41-49,9.DOI:10.15955/j.issn1000-3924.2023.06.08
一株野生树舌灵芝菌株鉴定及其液态发酵特性
Identification and liquid fermentation characteristics of a wild Ganoderma sp.strain
摘要
Abstract
In order to further enrich the fungal resources of Ganoderma,the fruiting bodies of Ganoderma collected from wild peach trees were identified by morphological observation and ITS sequence alignment.The mycelial biomass and characteristics of intracellular and extracellular metabolites in the shake flask fermentation broth were also analyzed.The results showed that the Ganoderma lucidum strain belongs to the Polyporaceae,Ganoderma,with the scientific name of G.applanatum.The sequence was uploaded to GenBank with the accession number OK037622.Liquid fermentation studies showed that its mycelial biomass was 15.16 g/L,the substrate glucose utilization rate was 77.63％,the content and concentration of intracellular and extracellular polysaccharides were 16.04 mg/g and 1.23 g/L,respectively.The intracellular metabolites mainly included sugar alcohols(45.07％),saccharides(40.05％),and fatty acids(13.33％).The extracellular metabolites mainly included amino acids(40.02％),saccharides(15.74％),sugar acids(12.09％),sugar alcohols(9.85％),fatty acids(6.28％),organic acids(5.82％),esters(4.34％),alcohols(2.83％),and nucleosides(2.79％).This study could provide strain preparation for the next step of Ganoderma research,and provide reference for subsequent development and application.关键词
药用菌/树舌灵芝/序列鉴定/液态发酵Key words
Medicinal fungi/Ganoderma applanatum/Sequence identification/Liquid fermentation分类
农业科学引用本文复制引用
冯杰,李晔,刘艳芳,唐传红,刘国辉,金高平,张劲松..一株野生树舌灵芝菌株鉴定及其液态发酵特性[J].上海农业学报,2023,39(6):41-49,9.基金项目
上海市人才发展资金(2019052) （2019052）
上海市农业科学院攀高计划(2022-2024年度) （2022-2024年度）
