同济大学学报（医学版）2023，Vol.44Issue(6)：785-791,7.DOI:10.12289/j.issn.1008-0392.23239
RA滑膜成纤维样细胞外泌体促进巨噬细胞向M1极化
M1 polarization is induced by exosomes derived from fibroblast-like synoviocytes from patients with rheumatoid arthritis
摘要
Abstract
Objective To investigate the role of exosomes derived from fibroblast-like synoviocytes from patients with rheumatoid arthritis(RA-FLS)in macrophage(Mø)polarization.Methods Synovial fluid and tissue from osteoarthritis(OA)patients(n=6)and rheumatoid arthritis(RA)patients(n=6)were collected,and the cytokines in joint fluid were detected using flow cytometry bead array(CBA)method.The expression of extracellular marker CD9 in the synovium was detected with immunohi-stochemical method.Primary synovial RA-FLS and OA-FLS were isolated,and the exosomes from cell-culture supernatant were identified by Western blotting and nanoparticle tracking analysis(NTA).The effects of OA-FLS and RA-FLS exosomes on the M1 and M2 phenotype genes were determined with real time fluorescence quantitative PCR(qRT-PCR)and CBA.Results The expressions of M1 type markers(IL-1 β,IL-6,IL-8),and M2 type marker(IL-10)in RA joint fluid were significantly higher than those in the OA group.High expression of CD9 was detected in RA synovium.RA-FLS was found to secrete more exosomes than OA-FLS.Elevated gene and protein expression levels of M1 markers were found in Mø treated with RA-FLS derived exosomes.Conclusion RA-FLS derived exosomes can induce M1 polarization in vitro.关键词
类风湿关节炎/滑膜成纤维细胞/巨噬细胞/外泌体/M1型极化Key words
rheumatoid arthritis/fibroblast-like synoviocytes/macrophage/exosome/M1 polarization分类
医药卫生引用本文复制引用
虞珊珊,李静,徐臻,成宇,宗明,范列英..RA滑膜成纤维样细胞外泌体促进巨噬细胞向M1极化[J].同济大学学报（医学版）,2023,44(6):785-791,7.基金项目
国家自然科学基金面上项目(81971535) （81971535）
上海市浦东新区卫生系统优秀青年医学人才培养计划(PWRq2020-21) （PWRq2020-21）
上海市"医苑新星"青年医学人才培养资助计划(YYXX202101) （YYXX202101）
