同济大学学报（医学版）2023，Vol.44Issue(6)：805-812,8.DOI:10.12289/j.issn.1008-0392.23065
抗人IL-17C单克隆抗体的制备和功能鉴定
Preparation and identification of monoclonal antibodies against human IL-17C
摘要
Abstract
Objective To prepare and identify monoclonal antibodies against human IL-17C.Methods The monoclonal antibodies against human IL-17C(mAbs)with hybridoma technique using six-week-old female BALB/c mice.The titers,cross-reactivity,specificity and subclass of the mAbs were determined.The applicability of the mAb was verified by immunoassay,and the mAb 7F10 was used as primary antibody for immunofluorescence and immunohistochemistry analysis.Results Nine hybridoma clones stably secreting mAb against human IL-17C were successfully screened out and named as 1F11,3A3,7F10,9B3,10C6,10A7,10B10,12F1 and 12F7,which all possessed significant receptor-blocking activity.Further studies showed that clone 7F10 and 10A7 could cause a cross-reaction between human and mouse IL-17C;and no cross-reactivity between mAb 7F10 or 10A7 and other members of the IL-17 family was observed.Both 7F10 and 10A7 were the IgG1 subclass antibodies,and the light chain was kappa type.The mAb 7F10 as primary antibody was successfully applied in immunofluorescence and immunohistochemistry assays.Conclusion The monoclonal antibodies against human IL-17C have been successfully prepared,among which 7F10 can be successfully applied in immunofluorescence and immunohistochemical detection,indicating that 7F10 may be used for diagnosis and treatment of IL-17C-associated diseases clinically.关键词
IL-17C/IL-17C抗体/单克隆抗体/自身免疫性疾病/肾脏疾病/小鼠Key words
IL-17C/IL-17C antibody/monoclonal antibody/autoimmune diseases/kidney diseases/mice分类
医药卫生引用本文复制引用
刘丽,张光远,张芳菲,王依凡,尹建永,汪年松,王锋..抗人IL-17C单克隆抗体的制备和功能鉴定[J].同济大学学报（医学版）,2023,44(6):805-812,8.基金项目
国家自然科学基金(81770741) （81770741）
