水生生物学报2024，Vol.48Issue(3)：372-383,12.DOI:10.7541/2024.2023.0159
黑水虻幼虫粉替代不同比例鱼粉对加州鲈生长、机体健康和肌肉品质的影响
REPLACING DIFFERENT PROPORTIONS OF FISHMEAL WITH HERMITIA ILLUCENS MEAL ON THE GROWTH,HEALTH AND MUSCLE QUALITY OF MICROPTERUS SALMOIDES
摘要
Abstract
Four diets with isonitrogenous and isolipidic(45％protein and 15％lipid)were prepared.The control group contained 30％fish meal(FM30),while the other three groups replaced 16.67％(FM25),33.33％(FM20)and 50％(FM15)fish meal with black soldier fly(Hermitia illucens)larvae meal respectively.The juvenile largemouth bass(Micropterus salmoides)was fed four diets in the indoor circulating water system for 8 weeks respectively.This study aimed to examine the impacts of replacing fish meal with H.illucens larvae meal on the growth performance,health and muscle quality of M.salmoides.The results showed that there was no significant difference in final body mass,weight gain rate(WGR),specific growth rate(SGR),and survival rate among the four groups(P＞0.05).However,the FM20 group exhibited a high WGR and SGR and a lowest feed conversion ratio(FCR),which was significantly lower than that of FM15 and FM25(P＜0.05).Partial replacement of fish meal with H.illucens larvae meal reduced the lipid content of the whole fish and liver,as well as the serum triglyceride content.Furthermore,it resulted in decreased levels of C14:0,C16:0,C18:1n-9 and C18:3n-3 in liver and DHA in muscle.Conversely,there was an elevation in C18:0,DHA,EPA and C22:5n-3 in the liver.These modifications had a marginal effect on amino acid composition in both muscle and liver.Compared with the control group,the contents of malondialdehyde in foregut and liver of the fish in FM20 group increased significantly(P＜0.05),while the activities of alkaline phosphatase in foregut and serum de-creased significantly(P＜0.05).The intestinal epithelium of the foregut was damaged with impaired intestinal villi and intestinal fold adhesion,the activities of lipase in foregut and the contents of crude lipid in muscle and crude protein in whole fish were the highest,accompanied with the highest DHA in liver of FM20 group.The above results revealed that the largemouth bass M.salmoides had an ability of endogenous highly unsaturated fatty acid(HUFA)biosynthesis.It is suggested that a 50％replacement of fish meal with H.illucens larvae meal(FM15)can be undertaken,with a minimum addition of 15％fish meal in diets of M.salmoides,which has no negative effects on the growth performance.However,the most suitable level is 33.33％replacement of fish meal.This study provides data for the development of nutritionally scientific and reasonable composition and low cost feed for M.salmoides.关键词
鱼粉替代/生长性能/脂肪酸/黑水虻幼虫/加州鲈Key words
Fish meal replacement/Growth performance/Fatty acid/Hermitia illucens/Micropterus salmoides分类
农业科技引用本文复制引用
游翠红,李鸿伟,周萌,林蠡,董烨玮,谭小红,黄燕华..黑水虻幼虫粉替代不同比例鱼粉对加州鲈生长、机体健康和肌肉品质的影响[J].水生生物学报,2024,48(3):372-383,12.基金项目
广东省2021年创新强校项目(KA21031H210) （KA21031H210）
广东省自然科学基金项目(2020A1515011252) （2020A1515011252）
广东省普通高校创新团队项目(2020KCXTD019) （2020KCXTD019）
广东省乡村振兴专项-农业科技发展及资源环境保护管理项目(2023KJ115)资助[Supported by Guang-dong Province 2021 Innovation for Stronger Schools Project(KA21031H210) （2023KJ115）
Natural Science Foundation of Guangdong Province(2020A1515011252) （2020A1515011252）
Innovative Team Projects of Ordinary Colleges and Universities in Guangdong Province(2020KCXTD019) （2020KCXTD019）
Guangdong rural revitalization project-agricultural science and technology development and resource and environmental protec-tion management project(2023KJ115)] （2023KJ115）
