水生生物学报2024，Vol.48Issue(3)：454-460,7.DOI:10.7541/2024.2023.0202
Sox8基因过表达诱导中华鳖ZW性腺的雄性分化
OVEREXPRESSION OF SOX8 GENE INDUCES MALE DIFFERENTIATION OF ZW GONAD OF CHINESE SOFT-SHELLED TURTLE,PELODISCUS SINENSIS
摘要
Abstract
Chinese soft-shelled turtle Pelodiscus sinensis is an important aquaculture species in China,the males have higher economic value due to their higher growth rate,larger body size and wider calipash compared to females.Under-standing the mechanism of sex differentiation is crucial for the production of mono-sex male offspring.However,the molecular mechanism of sex determination and gonadal differentiation in Chinese soft-shelled turtle is still unclear.Sox8 plays a pivotal role in male sex differentiation and testis maintenance in vertebrates.Knockdown of Sox8 led to the feminization of ZZ Chinese soft-shelled turtle(Pelodiscus sinensis).In this study,we employed a lentivirus-media-ted overexpression vector to elevate Sox8 expression in ZW gonads of Chinese soft-shelled turtles prior to sex diffe-rentiation.At stage 27,histological analysis and immunofluorescence staining for VASA,a germ cell marker,revealed that after the overexpression of Sox8,50％of ZW gonads possessed a medulla with testis-specific seminiferous cords containing germ cells.qRT-PCR and immunofluorescence results showed that the expression of female-specific genes Foxl2 and Cyp19a1 was significantly reduced,while the expression of Dmrt1,Sox9 and Amh,which regulate testicular development,was up-regulated.One month after hatching,the gonad of a Sox8-overexpressed ZW individual showed distinct male characteristics:the gonad contained a well-developed medulla and degenerated cortex,the germ cells were located in the sex cords,and SOX9 and DMRT1 proteins were abundantly expressed in the medulla whereas FOXL2 and AROM were not expressed at all.The results indicate that overexpression of Sox8 induces male differentiation in the ZW turtle embryos,suggesting the role of Sox8 in male differentiation in Chinese soft-shelled turtle.关键词
Sox8/过表达/雄性分化/中华鳖Key words
Sox8/Overexpression/Male differentiation/Pelodiscus sinensis分类
生物科学引用本文复制引用
庄天依,沈佳东,楼灵媛,王金霓,孙伟,肖玲,葛楚天..Sox8基因过表达诱导中华鳖ZW性腺的雄性分化[J].水生生物学报,2024,48(3):454-460,7.基金项目
国家自然科学基金(32373126和U22A20529) （32373126和U22A20529）
浙江省自然科学基金(LY23C190005) （LY23C190005）
浙江省高校基础科研经费项目(1741000555) （1741000555）
浙江省"生物工程"一流学科自设课题(ZS2022009)资助[Supported by the National Natural Science Foundation of China(32373126 and U22A20529) （ZS2022009）
Natural Science Foundation of Zhejiang Province(LY23C190005) （LY23C190005）
Basic Scientific Research Foundation of Zhejiang Provincial Universities （）
Zhejiang Provincial Top Discipline of Biological Engineering(Level A),Zhejiang Wanli University(ZS2022009)] （Level A）
