非天然氨基酸的应用及生物合成策略OACSCDCSTPCD

Unnatural amino acids(UAAs)are a special class of amino acids that are not constrained by genetic codons.They have unique spatial configurations and chemical properties,which provide new resources and opportunities in several fields.Currently,UAAs can be synthesized by both chemical synthesis and biosynthesis.Compared with chemical synthesis,biosynthesis is a promising synthesis with higher efficiency and stereoselectivity,lower cost and pollution.This paper reviews the research progress and application cases of UAAs in protein probes,enzyme engineering,antibody-drug conjugates,and antimicrobial peptides,etc.It shows how UAAs break through the limitations of natural amino acids and expand the structural and functional diversity of proteins.It also introduces the biosynthesis strategies of UAAs,including static and dynamic regulatory strategies in metabolic engineering and fermentation optimization strategies.Finally,it looks forward to the future development trends and challenges in the biosynthesis and application of unnatural amino acids,which will provide a reference and inspiration for the UAAs synthesis and development.