生物技术通报2023，Vol.39Issue(12)：43-55,13.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0648
非天然氨基酸的应用及生物合成策略
Application and Biosynthesis Strategies of Unnatural Amino Acids
摘要
Abstract
Unnatural amino acids(UAAs)are a special class of amino acids that are not constrained by genetic codons.They have unique spatial configurations and chemical properties,which provide new resources and opportunities in several fields.Currently,UAAs can be synthesized by both chemical synthesis and biosynthesis.Compared with chemical synthesis,biosynthesis is a promising synthesis with higher efficiency and stereoselectivity,lower cost and pollution.This paper reviews the research progress and application cases of UAAs in protein probes,enzyme engineering,antibody-drug conjugates,and antimicrobial peptides,etc.It shows how UAAs break through the limitations of natural amino acids and expand the structural and functional diversity of proteins.It also introduces the biosynthesis strategies of UAAs,including static and dynamic regulatory strategies in metabolic engineering and fermentation optimization strategies.Finally,it looks forward to the future development trends and challenges in the biosynthesis and application of unnatural amino acids,which will provide a reference and inspiration for the UAAs synthesis and development.关键词
非天然氨基酸/生物合成/代谢工程/非天然蛋白质Key words
unnatural amino acids/biosynthesis/metabolic engineering/unnatural protein引用本文复制引用
李海宁,张红兵,耿革霞,李冉,贾振华..非天然氨基酸的应用及生物合成策略[J].生物技术通报,2023,39(12):43-55,13.基金项目
河北省重点研发项目(22372803D),河北省科学院两院合作项目(2020031506) （22372803D）
