生物技术通报2023，Vol.39Issue(12)：81-89,9.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0698
基于抗体-适配体夹心生物传感器检测大肠杆菌O157:H7
Detection of Escherichia coli O157:H7 Based on Antibody Aptamer Sandwich Biosensor
摘要
Abstract
Escherichia coli O157:H7 is an important food borne pathogen related to public health.Antibody molecules are the most important effector molecules in the humoral immune response.They have a variety of biological activities.The main biological function is to specifically bind to the corresponding antigen.Aptamers are shorter DNA sequences synthesized in vitro,which can bind specifically by recognizing specific regions and targets.Based on the specific recognition function of antibody and aptamer,the capture effect and magnetic separation of immunomagnetic beads,and the construction of biosensor by polyclonal antibody and tailoring aptamer,real-time fluorescence quantitative polymerase chain reaction(qPCR)may achieve the detection of target at(8×103)-(8×106)CFU/mL.The limit of detection is 800 CFU/mL for quantitative detection.关键词
多克隆抗体/适配体/裁剪/免疫磁珠/大肠杆菌O157:H7Key words
polyclonal antibody/aptamer/tailoring/immunomagnetic beads/Escherichia coli O157:H7引用本文复制引用
侯炜辰,叶柯,李洁,张洋子,许文涛,朱龙佼,李相阳..基于抗体-适配体夹心生物传感器检测大肠杆菌O157:H7[J].生物技术通报,2023,39(12):81-89,9.基金项目
国家重点研发计划(2022YFF0607900),北京市景观休闲农业创新团队项目(BAIC09-2023),北京市科技计划(Z221100007122004) （2022YFF0607900）
