生物技术通报2023，Vol.39Issue(12)：99-108,10.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0580
RNAi技术在寄生蜂中的应用研究进展
Research Progress in the Application of RNAi Technology in Parasitoid Wasps
摘要
Abstract
RNA interference technology has been widely used for investigating functions of genes for more than 20 years since its inception.In Hymenoptera,parasitoid wasps are a group of important natural enemy against pests in agriculture and forestry.The inhibition of target gene expression relies on the delivery of dsRNA.However,the parasitoids develop on host insects,and interact with their hosts.This biological characteristic makes it challenging to conduct RNAi technology.The RNAi technology has already been conducted in the studies involved the molecular mechanisms of at least 13 species of parasitoid wasps,e.g.Nasonia vitripennis,Microplitis mediator,Cotesia vestalis,Pteromalus puparum,and Meteorus pulchbernis.This study reviewed the studies of the molecular mechanisms by using RNAi method,and summarized the factors influencing RNAi efficiency in parasitoid wasps,aiming to provide the reference for application of RNAi technology in parasitoid wasps.关键词
寄生蜂/RNA干扰/分子机制/RNAi效率Key words
parasitoid wasps/RNA interference/molecular mechanism/RNAi efficiency引用本文复制引用
张龙喜,吕琳,张欢欢,周金成,车午男,董辉..RNAi技术在寄生蜂中的应用研究进展[J].生物技术通报,2023,39(12):99-108,10.基金项目
国家自然科学基金项目(32172476),西藏自治区中央引导地方项目(XZ202301YD0042C),辽宁省教育厅科学研究经费项目(LJKZ0646) （32172476）
