RNAi技术在寄生蜂中的应用研究进展OACSCDCSTPCD
Research Progress in the Application of RNAi Technology in Parasitoid Wasps
RNA干扰技术问世至今 20 余年,已被广泛用于基因功能研究.膜翅目寄生蜂是农林害虫防治领域重要的天敌昆虫资源.RNAi技术对靶基因的昆虫沉默效果依赖于dsRNA的递送效率.寄生蜂幼期通过摄取寄主昆虫营养完成其生长发育,并与寄主昆虫发生相互作用,给RNAi技术的实施带来挑战.目前,RNAi技术已在丽蝇蛹集金小蜂(Nasonia vitripennis)、中红侧沟茧蜂(Microplitis mediator)、菜蛾盘绒茧蜂(Cotesia vestalis)、蝶蛹金小蜂(Pteromalus puparum)、斑痣悬茧蜂(Meteorus pulchricornis)等至少 13 种寄生蜂中开展.本文归纳了RNAi技术在寄生蜂基因调控方面的研究进展,并总结了在寄生蜂类群中影响RNAi效率的因素,以期为RNAi技术在寄生蜂类群中的应用提供参考.
RNA interference technology has been widely used for investigating functions of genes for more than 20 years since its inception.In Hymenoptera,parasitoid wasps are a group of important natural enemy against pests in agriculture and forestry.The inhibition of target gene expression relies on the delivery of dsRNA.However,the parasitoids develop on host insects,and interact with their hosts.This biological characteristic makes it challenging to conduct RNAi technology.The RNAi technology has already been conducted in the studies involved the molecular mechanisms of at least 13 species of parasitoid wasps,e.g.Nasonia vitripennis,Microplitis mediator,Cotesia vestalis,Pteromalus puparum,and Meteorus pulchbernis.This study reviewed the studies of the molecular mechanisms by using RNAi method,and summarized the factors influencing RNAi efficiency in parasitoid wasps,aiming to provide the reference for application of RNAi technology in parasitoid wasps.
张龙喜;吕琳;张欢欢;周金成;车午男;董辉
沈阳农业大学植物保护学院,沈阳 110866沈阳农业大学植物保护学院,沈阳 110866西藏自治区农牧科学院蔬菜研究所,拉萨 850032沈阳农业大学植物保护学院,沈阳 110866沈阳农业大学植物保护学院,沈阳 110866沈阳农业大学植物保护学院,沈阳 110866
寄生蜂RNA干扰分子机制RNAi效率
parasitoid waspsRNA interferencemolecular mechanismRNAi efficiency
《生物技术通报》 2023 (12)
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国家自然科学基金项目(32172476),西藏自治区中央引导地方项目(XZ202301YD0042C),辽宁省教育厅科学研究经费项目(LJKZ0646)
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