生物技术通报2023，Vol.39Issue(12)：109-117,9.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0518
单细胞染色质转座酶可及性高通量测序技术及其应用
Single Cell Assay for Transposase Accessible Chromatin with High-throughput Sequencing Technology and Its Applications
摘要
Abstract
Single-cell assay for transposase-accessible chromatin with high-throughput sequencing(scATAC-seq)is a high-throughput sequencing technique that utilizes transposase to investigate the chromatin accessibility at the single-cell level,which is of great significance for investigating the epigenetic regulation of the whole genome.It can reveal important information about chromatin organization from multiple perspectives,mapping the binding regions of transcription factors and the positioning of nucleosomes in cells.Currently,scATAC-seq has been widely applied in the fields of biology and medicine,primarily for generating open chromatin profiles,studying cell differentiation and development,elucidating disease pathogenesis,and exploring the tumor microenvironment.This article provides an overview of the development,data analysis,and relevant applications of scATAC-seq technology in studying the open chromatin regions at the single-cell level,aiming to provide insights for the investigation of chromatin accessibility at the single-cell whole-genome level,identification of cis-regulatory elements,and deciphering genetic regulatory networks,thereby contributing to advancements in life science research.关键词
scATAC-seq/数据分析/应用Key words
scATAC-seq/data analysis/application引用本文复制引用
赵若含,赵净颖,柏毅承,张瑞芳,贾俊静,豆腾飞..单细胞染色质转座酶可及性高通量测序技术及其应用[J].生物技术通报,2023,39(12):109-117,9.基金项目
国家自然科学基金项目(U1702232),云南省基础研究计划项目(202101AU070089) （U1702232）
