生物技术通报2023，Vol.39Issue(12)：136-147,12.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0512
52个马铃薯遗传多样性分析及SSR分子身份证构建
Genetic Diversity Analysis and Molecular Identity Card Construction by SSR Markers of 52 Solanum tuberosum L.Varieties(Lines)
摘要
Abstract
The evaluation on the genetic diversity of potato germplasm resources(Solanum tuberosum L.)is the prerequisite and basis for genetic breeding,which may provide breeding materials for exploring the genetic background of potato germplasm resources and breeding new varieties.The genetic relationship and diversity of 52 potato varieties(lines)was evaluated by SSR markers,and the fingerprint was constructed.Sixteen primers with abundant polymorphism and good stability were selected from 25 pairs of SSR primers,and 147 polymorphic bands were amplified by 16 pairs of SSR primers from 52 potato varieties(lines),and the average polymorphic band ratio was 64.71%.The average Nei's genetic diversity(H)and Shannon's index(I)were 0.14 and 0.24,respectively.At the similar genetic coefficient of 0.23,the 52 potato materials were divided into three categories by cluster analysis.Group I included 49 varieties(lines),46 varieties(lines)from North China,and 3 imported varieties(lines)from abroad,all of them were suitable for planting in North Shanxi province.Group II included two varieties,'Beifang 016'and'Jinshu 16',characterized by yellow potato skin and high dry matter content.Group III only included'Jinshu 15',and was not clustered according to geographical origin.At the genetic similarity coefficient of 0.36,group I can be divided into 7 subclasses.Based on the principle of identifying the most varieties with the least primers,C59,S25,C33 and S151 were used to distinguish all varieties tested,and the digital fingerprints and molecular identity cards based on SSR markers were constructed.The genetic diversity of potato germplasm resources can be evaluated by using SSR markers,and different potato varieties(lines)can be accurately identified and traced by molecular identification cards.关键词
马铃薯/品种(系)/遗传多样性/SSR标记/聚类分析/指纹图谱/分子身份证Key words
potato/varieties(lines)/genetic diversity/SSR markers/clustering analysis/fingerprints/molecular identity card引用本文复制引用
安苗,王彤彤,付逸婷,夏俊俊,彭锁堂,段永红..52个马铃薯遗传多样性分析及SSR分子身份证构建[J].生物技术通报,2023,39(12):136-147,12.基金项目
山西省基础研究计划项目(202203021211272),山西省重点研发计划项目(202102140601004),山西省现代农业产业技术体系建设专项资金资助(2023CYJSTX06-10),山西农业大学农学院育种工程专项(YZ2021-04),山西农业大学校市合作项目(2020HX51) （202203021211272）
