基于SSR标记的广东黄皮种质资源遗传多样性分析及分子身份证构建OACSCDCSTPCD

This work aims to reveal the genetic diversity and relationship among Guangdong wampee germplasms,and provide reference for the effective protection and efficient utilization of wampee germplasm resources.The wampee germplasms from Guangdong province were examined using 12 pairs of SSR primers to determine their genetic diversity.UPGMA was utilized for cluster analysis,while numerical and letter codes were assigned to create molecular identity cards.The results showed that the 12 pairs of primers generated a total of 43 alleles(Na),with an average of 3.583 alleles per locus in the 84 analyzed materials.The expected heterozygosity(He)ranged from 0.294 to 0.665,with a mean of 0.524.The polymorphism information content(PIC)ranged from 0.291 to 0.642,with a mean of 0.484.The Shannon information index(I)ranged from 0.567 to 1.096,with a mean of 0.913.The 84 germplasms were divided into six groups based on UPGMA clustering,and molecular identity was established for each examined germplasm resource.Guangdong wampee germplasm resources have high genetic diversity,while their clustering was not strictly conducted by their geographical origins.Based on the SSR genotyping,a digital and letter-based coding method was used to construct the molecular identity card for wampee resources.