生物技术通报2023，Vol.39Issue(12)：219-228,10.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0818
内生菌对干旱胁迫下甘蔗的生理响应及抗旱性评价
Physiological Response and Drought Resistance Evaluation of Endophytic Bacteria to Sugarcane Under Drought Stress
摘要
Abstract
This work aims to explore the physiological response of inoculated endophytic bacteria to sugarcane under drought stress and the effect of alleviating drought-tolerant,and to provide research basis for the development and utilization of sugarcane drought-tolerant functional strains and the promotion of drought resistance cultivation techniques in sugarcane.Four sugarcane endophytes with strong in vitro drought tolerance were selected as the target strains.Two soil water contents were set up in pot experiment,namely normal watering(CK:soil water content 25%-30%)and moderate drought stress(soil water content 10%-12.5%),and a total of 8 treatments were inoculated with single endophyte,combined endophyte and no endophyte,respectively.Physiological and biochemical indexes of sugarcane seedling stage were determined,and drought resistance of each treatment was evaluated comprehensively.Compared with CK,drought stress promoted the accumulation of proline(Pro),soluble protein(SP),soluble sugar(SS)and malondialdehyde(MDA)in sugarcane plants.The activity of superoxide dismutase(SOD)and peroxidase(POD)increased.The contents of leaf relative water content(RWC),water loss rate in vitro(RWL)and chlorophyll(ChI)decreased.Compared with the treatment without inoculation,the inoculation of endophytic bacteria effectively alleviated drought stress under drought stress,among which ZM strain inoculated alone(ZD)had the highest comprehensive evaluation value of drought resistance D,and the gray correlation analysis showed that SOD,ChI,RWC and Pro were closely related to the D values of each treatment.ZM strain inoculation has the best effect on alleviating drought stress of sugarcane seedling,and can improve the drought resistance of sugarcane seedling by increasing the contents of ChI,RWC,Pro and SOD activity.关键词
甘蔗/接种内生菌/抗旱性/生理响应/综合性评价Key words
sugarcane/inoculate endophytic bacteria/drought resistance/physiological response/comprehensive evaluation引用本文复制引用
罗艳菊,谢林艳,邹清林,李四杰,刘涵,刘鲁峰,何丽莲,李富生..内生菌对干旱胁迫下甘蔗的生理响应及抗旱性评价[J].生物技术通报,2023,39(12):219-228,10.基金项目
云南省重大科技专项(202202AE090021),云南省教育厅科学研究基金(2022J0286),云南省作物生产与智慧农业重点实验室专项(202105AG070007) （202202AE090021）
