生物技术通报2023，Vol.39Issue(12)：229-236,8.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0152
贝莱斯芽孢杆菌ZHX-7的分离鉴定及抑菌促生效果
Isolation and Identification of Bacillus velezensis ZHX-7 and Its Antibacterial and Growth-promoting Effects
摘要
Abstract
In order to obtain highly effective biocontrol microorganism for controlling peanut diseases,using Aspergillus niger as the indicator,a highly effective biocontrol strain ZHX-7 was screened from soil samples by agar disk dilution and dual-culture assay.The soil samples were collected from the rhizosphere of diseased and healthy peanut plants in continuous cropping field in Laixi,Shandong province.The strain ZHX-7 was identified as Bacillus velezensis based on the morphological characteristics,16S rDNA and gyrB sequence analysis.The bacterial suspension,sterile fermentation broth and volatile gas of ZHX-7 significantly inhibited the growth of Sclerotium rolfsii,Fusarium neocosmosporiellum,Phoma arachidicola and Alternaria alternata.The antagonistic experiment results showed that the inhibition rates of ZHX-7 suspension against the growth of S.rolfsii,F.neocosmosporiellum,P.arachidicola and A.alternata were 64.40%,70.37%,77.43%and 65.32%,respectively.In the sterile fermentation broth of ZHX-7 diluted 10 times with PDA medium,the inhibition rates of ZHX-7 against the growth of S.rolfsii,F.neocosmosporiellum,P.arachidicola and A.alternata were 67.81%,52.16%,23.52%and 69.93%,respectively.The double dish test showed that the inhibition rates of ZHX-7 volatile gas against the growth of S.rolfsii,F.neocosmosporiellum,P.arachidicola and A.alternata were 86.82%,35.40%,81.70%and 44.19%,respectively.ZHX-7 produced secondary metabolites such as protease,cellulose and amylase.In addition,under the condition of pot culture in greenhouse,ZHX-7 fermentation broth significantly promoted the growth of peanut.Compared with the control,the fresh mass and dry mass increased by 31.24%and 26.47%,respectively.ZHX-7 fermentation broth improved the resistance of peanut to S.rolfsii,and the control effect reached 51.76%.In conclusion,a strain of B.velezensis ZHX-7 screened in this study may inhibit a variety of peanut pathogenic fungi and promote peanut growth at the same time.It is a multifunctional strain with broad antagonistic spectrum against peanut diseases,and high potential for biocontrol application.关键词
贝莱斯芽孢杆菌/花生病害/分离鉴定/抑菌率/生物防治/促生作用/白绢病菌Key words
Bacillus velezensis/peanut diseases/isolation and identification/antibacterial rate/biological control/growth-promoting effect/Sclerotium rolfsii引用本文复制引用
李莹,宋新颖,何康,郭志青,于静,张霞..贝莱斯芽孢杆菌ZHX-7的分离鉴定及抑菌促生效果[J].生物技术通报,2023,39(12):229-236,8.基金项目
国家自然科学基金项目(31901940,32202326,31972967),山东省自然科学基金项目(ZR2021QD153,ZR2020QC131),青岛市自然科学基金项目(23-2-1-46-zyyd-jch),山东省农业科学院农业科技创新工程(CXGC2021B33,CXGC2023A46) （31901940,32202326,31972967）
