生物技术通报2023，Vol.39Issue(12)：276-286,11.DOI:10.13560/j.cnki.biotech.bull.1985.2023-0710
珊瑚状猴头菇多糖的提取及其体外抗氧化活性分析
Extraction of Polysaccharide from Hericium corallinum and Analysis on Its in vitro Antioxidant Activity
摘要
Abstract
Hericium corallinum,a newly cultivated and domesticated species,has a high content of polysaccharides with physiologically active properties such as antioxidation.Using the polysaccharide extraction rate as a benchmark,the response surface methodology was utilized to optimize the hot water extraction process of H.corallinum polysaccharides(HCP).UV,FT-IR and X-ray diffraction were used to preliminarily characterize HCP,and its antioxidative activity in vitro was measured.The results showed that the optimal process parameters for extracting HCP were an extraction temperature of 93℃,an extraction time of 2.0 h,a material-to-liquid ratio of 1∶33(g/mL),and a particle size of 80 mesh.The characterization results showed that HCP contained a small amount of proteins and nucleic acids,with typical absorption peaks of polysaccharide compounds and pyranose ring structure,and showed the presence of crystalline and non-crystalline structures.In vitro antioxidant experiments showed that HCP had good scavenging effects on DPPH,ABTS+ and·OH radicals,and their EC50 values were 0.663 mg/mL,0.767 mg/mL and 0.952 mg/mL,respectively.HCP has good antioxidant activity,and the results lay a research foundation for further exploring the antioxidant activity and other effects of coralline HCP in vivo.关键词
珊瑚状猴头菇多糖/提取工艺/响应面法/结构/抗氧化Key words
polysaccharide of Hericium coralloides/extraction process/response surface methodology/structure/antioxidant activity引用本文复制引用
涂晓媛,褚路路,王冕,陈炳智,江玉姬..珊瑚状猴头菇多糖的提取及其体外抗氧化活性分析[J].生物技术通报,2023,39(12):276-286,11.基金项目
安职院重点基金(N202304),福建农林大学创新基金(KFA20116A) （N202304）
