生殖医学杂志2024，Vol.33Issue(1)：29-34,6.DOI:10.3969/j.issn.1004-3845.2024.01.005
IVF/ICSI-ET术后早期妊娠丢失患者绒毛组织拷贝数变异异常的高危因素分析
Analysis of risk factors for abnormal copy number variation of villus tissue of patients with early pregnancy loss after IVF/ICSI-ET
摘要
Abstract
Objective:To investigate the risk factors of abnormal villus copy number variation(CNV)in patients with early pregnancy loss(EPL)who underwent fresh IVF/ICSI-ET,in order to provide the basis for genetic counseling. Methods:A retrospective cohort study was used to select the IVF/ICSI patients with abnormal CNV results of EPL villus tissue in Reproductive Medicine Center,General Hospital of Ningxia Medical University from June 2020 to December 2022 as the case group(n=29).The patients with normal live birth during the same period were randomly selected as normal control group(n=60).The general data,clinical and laboratory indexes of the two groups were compared,and the composition and proportion of abnormal CNV in aborted villi tissue of the case group were analyzed and discussed. Results:The age[(33.45±3.78)years vs.(29.52±4.27)years]and spontaneous abortion times[(0.41±0.73)vs.(0.05±0.22)]in case group were significantly higher than those in the control group(P＜0.05).Anti-Mullerian hormone(AMH)level in case group was significantly lower than that of control group[(2.49±2.05)ng/ml vs.(4.03±2.67)ng/ml](P＜0.05).The FSH level[(6.63± 6.31)U/L vs.(3.10±2.13)U/L]and LH level[(2.28±1.85)U/L vs.(1.26±0.79)U/L]on gonadotropin(Gn)initiation day,LH level on HCG day[(3.05±3.28)U/L vs.(1.27±1.14)U/L]in the case group were significantly higher than those control group(P＜0.05).In the CNV-sequence results of the villi tissues of the case group,chromosome number abnormalities accounted for 72.41％,structural abnormalities accounted for 27.59％.Autosomal trisomy accounted for 55.17％,among which trisomy16 and trisomy 22 were the most common(17.24％ and 10.34％,respectively). Conclusions:Advanced age and decreased ovarian reserve function may be the main factors leading to abnormal chromosome karyotype of embryos in patients with early pregnancy loss.关键词
IVF/ICSI/绒毛染色体/拷贝数变异/非整倍体Key words
IVF/ICSI/Villous chromosome/Copy number variation/Aneuploidy分类
医药卫生引用本文复制引用
黄蕾,何蕊,许晓雪,闫慧,赵君利,哈灵侠,刘春莲..IVF/ICSI-ET术后早期妊娠丢失患者绒毛组织拷贝数变异异常的高危因素分析[J].生殖医学杂志,2024,33(1):29-34,6.基金项目
宁夏回族自治区自然科学基金立项项目(2023AAC03599) （2023AAC03599）
