天然气工业2023，Vol.43Issue(12)：152-160,9.DOI:10.3787/j.issn.1000-0976.2023.12.016
微生物菌群的甲烷氧化效能及吸附煤体特性
Methane oxidizing capacity and coal adsorbing characteristics of microbial flora
摘要
Abstract
Methane-oxidizing bacteria gets energy by consuming methane,so it has wide application value in environmental methane emission reduction,but its application in coal mines underground is still in the stage of initial research.In order to promote the application of microbial technologies in coal mines and improve the treatment effect of coalbed methane,this paper selects microbial flora from the natural environment and acclimates them.Then,the microenvironmental change and gas consumption characteristics of the bacterial solution during the growth process are monitored with the experimental culture device,and the dynamic change of contact angle of pure water,culture medium and bacterial solution on the surface of different coal samples is investigated by means of contact angle measuring device and theoretical analysis.Finally,the influences of minerals in QC and XA coal samples,the former anthracite coal from Jincheng coal mine in Shanxi province and the latter coking coal from Xin'an Coal Mine in Henan Province,on wetting angle are analyzed by virtue of Fourier Transform Infrared Spectroscopy(FTIR).And the following research results are obtained.First,the selected microbial flora has strong methane oxidizing capacity.The results of two groups of experiment indicate that the methane degradation rate can reach 71.19％ and 84.63％ within 15 days,and the oxygen reduction rate can reach 100％.Second,the OD600 value of microbial growth density in the bacterial solution increases by 30 times,and the growth is the most vigorous from the 6th day to the 8th day.The pH value of bacterial solution increases from 6.32 to 7.25,and the ORP decreases from 210 mV to 93 mV.Third,compared with pure water and culture medium,bacterial solution has stronger wettability on the surface of coal and the smallest equilibrium contact angle on the surface of two kinds of coal samples.XA coal samples contain many hydrophilic minerals,and their equilibrium contact angles in three kinds of solution are smaller than that of QC coal samples.In conclusion,methane-oxidizing bacteria flora has high adaptability and application value in coal mines underground,indicating that microbial technologies have certain advantages in coal mine methane prevention and control and are of great significance for the safe production of coal mines.关键词
甲烷减排/甲烷氧化菌/润湿角/甲烷氧化/消耗特征/甲烷降解率/生长特性Key words
Methane emission reduction/Methane-oxidizing bacteria/Wetting angle/Methane oxidation/Consumption characteristics/Methane degradation rate/Growth characteristics引用本文复制引用
周银波,张瑞林,魏建平,田坤云,宫伟东,赵双,石涵锋..微生物菌群的甲烷氧化效能及吸附煤体特性[J].天然气工业,2023,43(12):152-160,9.基金项目
国家自然科学基金项目"煤的趋化作用及其改造煤吸附甲烷特性实验研究"(编号:52174168),河南省高校科技创新团队计划和创新人才支持计划项目"煤矿瓦斯治理与煤层气开发"(编号:221RTSTHN009)、"煤矿甲烷绿色减排"(编号:24HAS-TIT019),河南省科协青年人才托举工程项目"甲烷氧化菌在煤矿甲烷绿色减排和治理工作中的应用基础研究"(编号:2023HYTP014),河南省高校重点科研项目"二氧化碳驱替煤层瓦斯技术的气体储运特征研究"(编号:23A620004). （编号:52174168）
