电针对心肌缺血再灌注损伤大鼠心室结构和功能的影响OACSTPCD

Objective To observe the effect of electroacupuncture(EA)on changes of ventricular structure and function in rats with myocardial ischemia-reperfusion injury(MIRI),so as to explore its potential mechanisms un-derlying improvement of ventricular remodeling after MIRI.Methods Forty male SD rats were randomly divided into 4 groups:sham operation group,model group,EA group and medication(sacubactril valsartan,LCZ696)group,with 10 rats in each group.The MIRI model was established by ligation of the left anterior descending coronary artery and reperfu-sion.EA(2 Hz/100 Hz,2 mA)was applied to bilateral"Neiguan"(PC6)for 20 min,once every other day for 21 d.Rats of the medication group received gavage of LCZ696(60 mg·kg-1·d-1).After the intervention,echocardiography was used to detect the ejection fraction(EF)and fractional shortening(FS)of the left ventricle,and the contents of serum tumor necrosis factor-α(TNF-α),vascular cell adhesion molecule-1(VCAM-1)and intercellular cell adhesion molecule-1(ICAM-1)were assayed by enzyme-linked immunosorbent assay.The pathological changes of myocardial tissue were observed after HE staining.The Masson staining was used to evaluate the myocardial collagen deposition and myocar-dial fibrosis.The mRNA expression levels of collagen Ⅰ and Ⅲ and connective tissue growth factor(CTGF)in the myo-cardial tissue were detected by quantitative real-time PCR,and the expression levels of IL-1β and IL-18 were detected by Western blot.Results In contrast to the sham operation group,the EF and FS levels of the left ventricle were ob-viously decreased(P<0.001),while the contents of serum TNF-α,VCAM-1 and ICAM-1,the proportion of myocardial fibrosis area,the mRNA expression levels of myocardial collagen Ⅰ,collagen Ⅲ and CTGF,the expression levels of IL-1β and IL-18 were significantly increased(P<0.001,P<0.0001,P<0.05,P<0.01)in the model group.Compared with the model group,the EF and FS levels were remarkably increased(P<0.01),whereas the contents of serum TNF-α,VCAM-1 and ICAM-1,the proportion of myocardial fibrosis area,the mRNA expression levels of myocardial collagen Ⅰ,collagen Ⅲ and CTGF,and the expression levels of IL-1β and IL-18 were significantly down-regulated(P<0.001,P<0.01,P<0.05)in both the medication and EA groups.No significant differences were found between the EA and medi-cation groups in all the indexes mentioned above.Conclusion EA can improve the left-ventricular fibrosis and func-tion,delay or reverse ventricular remodeling in MIRI rats,which may be related to its functions in down-regulating myo-cardial inflammatory response and mRNA expression levels of myocardial collagen Ⅰ,collagen Ⅲ and CTGF.