针刺研究2024，Vol.49Issue(1)：30-36,46,8.DOI:10.13702/j.1000-0607.20221174
电针对慢性炎性痛小鼠炎性反应和免疫细胞的影响
Effect of electroacupuncture on inflammatory response and immune cells in mice with chronic inflammatory pain
摘要
Abstract
Objective To observe the effects of electroacupuncture(EA)on local inflammatory mediators and macrophage polarization,and immune cells in the spleen of mice with chronic inflammatory pain induced by complete Freund's adjuvant(CFA)in the hind paw,so as to investigate the immunoinflammatory regulatory mechanisms of EA in relieving pain and swelling in mice with chronic inflammatory pain.Methods Thirty C57BL/6 mice were randomly di-vided into control,model,and EA groups,with 10 mice in each group.Chronic inflammatory pain model were estab-lished by subcutaneous injection of 20 μL CFA solution in the left hind paw for 7 consecutive days.After modeling,mice in the EA group received EA at bilateral"Zusanli"(ST36)for 20 min(2 Hz/100 Hz,1 mA)once a day for 18 consecu-tive days.Mechanical pain threshold,heat pain thresholds,and paw thickness were measured before and after mode-ling,and after interventions.Western blot was used to detect the expression of tumor necrosis factor-alpha(TNF-α),interleukin(IL)-1β,and NOD-like receptor protein 3(NLRP3)in the paw tissue.Immunohistochemistry was used to de-tect the positive expression of M1-type macrophage marker inducible nitric oride synthase(iNOS)and M2-type marker CD206 in the paw,and flow cytometry was used to detect the proportion of F4/80+ CD11b+ macrophages,Ly6G+ CD11b+ neutrophils,and CD25+ Foxp3+ regulatory T cells(Treg)in the spleen.Results Compared with the control group,mechanical pain and heat pain thresholds were significantly reduced(P<0.0001),while paw thickness,expres-sions of IL-1β,TNF-α,and NLRP3 in the paw,and positive expression of M1 macrophage marker iNOS in the paw,the proportions of macrophages and neutrophils in the spleen were significantly increased(P<0.0001,P<0.001)in the model group.Compared with the model group,mechanical pain threshold and heat pain thresholds,CD206 positive expression in the paw,and Treg cell proportion in spleen were significantly increased(P<0.01),while paw thickness,the expressions of IL-1β,TNF-α and NLRP3 in the paw,as well as the positive expression of M1 macrophage marker iNOS in the paw,the proportions of macrophages and neutrophils in the spleen were significantly reduced(P<0.001,P<0.01,P<0.05)in mice of the EA group after intervention.Conclusion EA may alleviate pain and swelling in mice with chronic inflammatory pain by regulating the numbers of macrophages,neutrophils,and Treg cells,as well as promoting M2 polarization of local macrophages and inhibiting the release of pro-inflammatory cytokines.关键词
电针/慢性炎性痛/抗炎/镇痛/免疫细胞/巨噬细胞极化Key words
Electroacupuncture/Chronic inflammatory pain/Anti-inflammation/Analgesia/Immune cells/Macrophage polarization引用本文复制引用
杨嘉丽,施珺菁,马乃骐,江敏娇,彭柔,陈栎遥,于美玲,卢圣锋..电针对慢性炎性痛小鼠炎性反应和免疫细胞的影响[J].针刺研究,2024,49(1):30-36,46,8.基金项目
国家自然科学基金项目(No.81774210) （No.81774210）
江苏省"青蓝工程"中青年学术带头人项目(No.2020) （No.2020）
江苏省"六大人才高峰"项目(No.YY-033) （No.YY-033）
张家港市青年科技项目(No.ZJGQNKJ202014) （No.ZJGQNKJ202014）
