摘要
Abstract
Objective To evaluate the value of combination detection of serological Epstein-Barr virus(EBV)markers Rta protein IgG antibody(Rta/IgG),capsid antigen IgA antibody(VCA/IgA),early antigen IgA antibody(EA/IgA)in nasopharyngeal carcinoma(NPC)screening in Linyi area.Methods Clinical data of 15 873 subjects in physical examination group,1 800 subjects in screening group with nasopharyngeal symptoms and 48 subjects in confirmed NPC group in People's Hospital of Linyi City from October 2019 to October 2022,were collected.The serum levels of EBV Rta/IgG,VCA/IgA and EA/IgA were detected by euzymelinked immunosorbent assay in all the patients.Statistical analyses were performed with receiver operating characteristic curve to evaluate the diagnostic values of the three indexes and combined detection for NPC.Results The positive rates of Rta/IgG,VCA/IgA and EA/IgA in the confirmed NPC group were 72.90％(35/48),85.41％(41/48),85.41％(41/48),respectively.In the screening group,their positive rates were 17.17％(309/1 800),27.06％(487/1800),and 17.72％(319/1 800),and those in the physical examination group were 10.34％(1 642/15 873),19.30％(3 070/15 873)and 9.96％(1 581/15 873),respectively.Their positive rates of the confirmed NPC group were significantly higher than those of the screening group(x2=95.918,78.029 and 136.589,all P＜0.01)and those of the physical examination group(x2=198.823,132.894 and 297.787,all P＜0.01).The differences were all statistically significant.Sensitivities of Rta/IgG,VCA/IgA and EA/IgA detection were 72.90％,85.41％,and 85.41％,respectively,while the specificities were 89.0％,79.9％and 89.2％,respectively.The sensitivity and specificity of the combination detection of the three antibodies were 95.83％(46/48)and 99.12％(17 518/17 673),respectively.And the area under the ROC of the combination detection was 0.949,which was higher than those of the three antibodies alone(0.846,0.901 and 0.916).Conclusion The combined detection of Rta/IgG,VCA/IgA and EA/IgA can expand the detection range of the antigen expression in the latency and lysis period of EBV,which has a good complementary effect and significantly improves the detection rate of NPC.关键词
鼻咽肿瘤/疱疹病毒4型,人/抗体/诊断Key words
Nasopharyngeal neoplasms/Herpesvirus 4,Human/Antibodies/Diagnosis分类
医药卫生
