中国烟草科学2023，Vol.44Issue(6)：12-20,9.DOI:10.13496/j.issn.1007-5119.2023.06.003
长期不同施肥制度下植烟土壤氮组分变化及影响因素分析
Analysis of Changes in Nitrogen Components and Influencing Factors in Tobacco Soil under Different Long-term Fertilization Regimes
摘要
Abstract
Five treatments were analyzed to investigate the changes in soil N fractions and their relationship with soil chemical properties,enzyme activities and gene abundance in different fertilization treatments,including no fertilization(CK),single application of inorganic fertilizer(NPK),single application of organic fertilizer(OM),combined application of inorganic fertilizer and organic fertilizer(NPKO),and combined application of inorganic fertilizer and winter green manure(NPKG).The results showed that:(1)Compared with CK and NPK,the OM and NPKO treatments significantly increased the contents of soil total nitrogen,microbial biomass nitrogen,water-soluble organic nitrogen and nitrate nitrogen.(2)Compared with CK,the OM and NPKO treatments significantly increased whole carbon,microbial biomass carbon and effective phosphorus contents of the soil;Compared with NPK,the OM treatment had a significant effect on carbon composition and available phosphorus contents.(3)Compared with CK,the OM,NPKO and NPKG treatments significantly increased soil ammonia monooxygenase,nitrate reductase and urease activities and functional gene abundance of amoB,nirK,nirS and nosZ by 9.47％～31.13％,46.46％～1613.48％,50.41％～323.72％,108.19％～611.02％,64.19％～252.15％,153.20％～373.21％and 56.90％～299.47％,respectively,while NPK significantly reduced the activities of soil nitrate reductase,urease and leucine aminopeptidase and amoA and nirK functional gene abundance.(4)Soil nitrate reductase,urease and nitrous oxide reductase were the main influencing factors for the change of soil nitrogen composition,and there was a highly significant correlation between soil nitrate reductase and soil N fractions(p＜0.01).Under long-term fertilization conditions,the activities of key enzymes related to soil nitrogen conversion was a key factor affecting nitrogen conversion in tobacco-planting soils.关键词
植烟土壤/氮组分/环境因子/土壤酶活性/基因丰度/结构方程/冗余分析Key words
tobacco-planting soils/nitrogen components/environmental factors/soil enzyme activity/gene abundance/model of structural equations/Redundant analysis分类
农业科技引用本文复制引用
肖鑫,丛萍,徐艳丽,况帅,杨如意,战徊旭,姜淑祯,张燕,董建新..长期不同施肥制度下植烟土壤氮组分变化及影响因素分析[J].中国烟草科学,2023,44(6):12-20,9.基金项目
中央级公益性科研院所基本科研业务费专项(1610232023011) （1610232023011）
山东临沂烟草有限公司技术开发项目(2021371300260162) （2021371300260162）
