中国烟草科学2023，Vol.44Issue(6)：44-52,9.DOI:10.13496/j.issn.1007-5119.2023.06.007
湖南烟草弯孢菌叶斑病和炭疽病病原鉴定及生物学特性研究
Identification and Biological Characterization of Curvularia Leaf Spot and Anthracnose Diseases on Tobacco in Hunan Province
摘要
Abstract
From 2020 to 2023,two foliar diseases suspected to be curvularia leaf spot and anthracnose were found on tobacco in Chenzhou City,Hunan Province.The objective of this study was to clarify the pathogens and provide theoretical reference for scientific and effective control of the diseases.The fungal pathogens were isolated from symptomatic leaves by tissue isolation and the diseases were identified to be leaf spot and anthracnose by both morphological observation and Koch's postulates.Combined with the morphological characteristics and ITS based phylogenetic analysis,the pathogen of the tobacco leaf spot was determined to be Curvularia trifolii,while the pathogen of the tobacco anthracnose was identified as Colletotrichum karstii using both the morphological and molecular characteristics by ITS,ACT,TUB,CHS-1 and GPDH gene regions.The study of biological characteristics showed that the optimum temperature for mycelium growth of C.trifolii was 25 ℃,the optimum pH was 7,and it was most favorable for mycelium growth when soluble starch was used as carbon source and beef powder as nitrogen source.The optimum temperature for mycelium growth of C.karstii was 25 ℃,the optimum pH was 7,and it was most favorable for mycelium growth when glucose was used as carbon source and beef powder as nitrogen source.To our knowledge,this is the first time that these two diseases are reported in Hunan Province of China.Up to now,there is no report of C.karstii causing disease in flue-cured tobacco in China.The results can provide a basis for the scientific and effective prevention and control of these diseases.关键词
车轴草弯孢菌/喀斯特炭疽菌/弯孢菌叶斑病/炭疽病/烟草病原菌Key words
Curvularia trifolii/Colletotrichum karstii/Curvularia leaf spot/anthracnose/tobacco pathogens分类
农业科技引用本文复制引用
肖艳松,李思军,吴文信,刘天波,周向平,黎萍,钟杰..湖南烟草弯孢菌叶斑病和炭疽病病原鉴定及生物学特性研究[J].中国烟草科学,2023,44(6):44-52,9.基金项目
湖南省郴州市烟草公司烟草农业科技创新项目(CZYC2022JS03) （CZYC2022JS03）
中国烟草总公司湖南省公司科技重点项目(HN2021KJ01) （HN2021KJ01）
湖南省郴州市烟草公司烟草农业科技创新项目(CZYC2023JS05) （CZYC2023JS05）
