中国烟草科学2023，Vol.44Issue(6)：75-83,9.DOI:10.13496/j.issn.1007-5119.2023.06.011
HS-GC-IMS结合ROAV分析烤烟烟叶"糯米香"特征成分
Analysis of Characteristic"Glutinous Rice"-like Aroma-active Odorants in Flue-cured Tobacco Leaves Based on HS-GC-IMS Combined with ROAV
摘要
Abstract
In order to accurately identify the potent odorants in flue-cured tobacco(FCT)leaves with"glutinous rice"-like characteristic,different Yunyan 87 tobacco leaves with or without typical"glutinous rice"-like characteristic from Yunnan province were used as materials in this study.Firstly,volatile compositions and their relative content was analyzed by headspace-gas chromatography-ion migration spectroscopy(HS-GC-IMS).Then,the differential volatile markers between typical"glutinous rice"-like and non-"glutinous rice"-like tobacco leaves samples and the key chemical contributors to the"glutinous rice"-like characteristic were identified based on the relative odor activity value(ROAV)combined with orthogonal partial least squares discriminant analysis(OPLS-DA).A total of 57 common odorants(including monomers and dimers)were identified.2-Acetyl-1-pyrroline("glutinous rice"-like),benzene acetaldehyde(fruity),nonanal(citrus-like),and 2,3-butanedione(sweet,buttery)are characterized as differential flavor compounds between typical"glutinous rice"-like and non-"glutinous rice"-like tobacco leaves samples,among which 2-acetyl-1-pyrroline is a key contributor to"glutinous rice"-like aroma,the other three compounds play an important role in modifying"glutinous rice"-like characteristic flavor of FCT.The results herein would provide chemical basis for the accurate regulation and the evaluation method development for"glutinous rice"-like characteristic odorants in FCT.关键词
烤烟/糯米香/挥发性关键风味化合物/顶空-气相色谱-离子迁移谱/相对气味活度值/2-乙酰基-1-吡咯啉Key words
flue-cured tobacco/"glutinous rice"-like aroma/volatile key odorants/headspace-gas chromatography-ion migration spectroscopy(HS-GC-IMS)/relative odor activity value(ROAV)/2-acetyl-1-pyrroline分类
轻工业引用本文复制引用
李舒畅,李军,高宪辉,刘文霖,常诗瑜,欧泽洁,庞雪莉,孔凡玉..HS-GC-IMS结合ROAV分析烤烟烟叶"糯米香"特征成分[J].中国烟草科学,2023,44(6):75-83,9.基金项目
中国农业科学院科技创新工程(ASTIP-TRIC07) （ASTIP-TRIC07）
中国烟草总公司云南省公司科技项目(2021530000241011) （2021530000241011）
中国烟草总公司贵州省公司科技项目(2021520000240044) （2021520000240044）
