中国烟草科学2023，Vol.44Issue(6)：100-105,6.DOI:10.13496/j.issn.1007-5119.2023.06.014
工业发酵雪茄烟叶霉变微生物的分离鉴定及生物学特性研究
Isolation,Identification and Biological Characterization of Mold Species from Industrial Fermented Cigar Tobacco Leaves
摘要
Abstract
To identify the mold species during the industrial fermentation process of cigar tobacco leaves,fungi were isolated and purified from the surface of moldy cigar tobacco leaves,and were identified with the combination of morphological and molecular techniques.The biological characteristics of mold were also investigated.The results showed that the main mold causing moldiness of cigar tobacco leaves were Aspergillus in this study,and three strains of mold were identified as A.tabacinus,A.flavus and A.montevidensis.The growth rate of the three mold strains were in the order of A.flavus＞A.tabacinus＞A.montevidensis on different culture media.All three mold strains could grow at 25 ℃ to 35 ℃,only A.flavus could grow at 40 ℃,and none of the strains could grow at 45 ℃ or higher temperature.The optimal pH for A.flavus was 4,while A.tabacinus and A.montevidensis were most suitable for pH 8-9.All three mold strains were sensitive to UV radiation,and after 15 minutes of exposure,the mortality rate was over 90％.Overall,the results from this study indicated that the three types of Aspergillus that cause cigar tobacco leaf mold during industrial fermentation are A.tabacinus,A.flavus and A montevidensis.The rational use of ultraviolet irradiation is important for mold control and prevention.关键词
雪茄/霉变/微生物/鉴定/生物学特性Key words
cigar/mold/microorganisms/identification/biological characteristics分类
轻工纺织引用本文复制引用
刘琳琳,芦柯,胡延奇,常月勇,李艳,时永楠,李萌,李晓..工业发酵雪茄烟叶霉变微生物的分离鉴定及生物学特性研究[J].中国烟草科学,2023,44(6):100-105,6.基金项目
中国烟草总公司重大科技项目(110202201034) （110202201034）
山东中烟工业有限责任公司科技项目(KJXM-GS-202202006) （KJXM-GS-202202006）
