中国医药科学2023，Vol.13Issue(24)：13-16,4.DOI:10.20116/j.issn2095-0616.2023.24.03
rhG-CSF联合PEG-rhG-CSF治疗小鼠中性粒细胞减少症的研究
Study on the rhG-CSF combined with PEG-rhG-CSF in the treatment of neutropenia in mice
摘要
Abstract
Objective To explore the therapeutic effect of recombinant human granulocyte colony stimulating factor(rhG-CSF)combined with pegylated recombinant human granulocyte colony stimulating factor(PEG-rhG-CSF)on neutropenia(NP)in mice.Methods A total of 24 Balb/c mice were evenly divided into the CTL group,Model group,PEG-rhG-CSF group and rhG-CSF+PEG-rhG-CSF group.The NP model was established in mice induced by the cyclophosphamide.After administration,the weight of the spleen,the number of colony-forming unit-spleen(CFU-S)on the surface of the spleen,and the number of bone marrow nucleated cells(BMNCs)were observed.Blood routine tests were conducted for the number of neutrophils in the peripheral blood of mice after 3-9 days.Flow cytometry was used to detect the content of CD11b+Ly6G+ cell populations in the peripheral blood of mice and the expression of CXCR4+ in bone marrow.Results Compared with the Model group,the weight of the spleen,CFU-S number,BMNCs number,CD11b+Ly6G+ cell population in peripheral blood,and CXCR4+ expression in the bone marrow of mice in the PEG-rhG-CSF group and rhG-CSF+PEG-rhG-CSF group increased significantly,with statistically significant differences(P<0.05).Compared with the PEG-rhG-CSF group,the rhG-CSF+PEG-rhG-CSF group showed a more significant increase in CFU-S number,BMNCs number,CD11b+Ly6G+ cell population in peripheral blood,and CXCR4+ expression in the bone marrow,with statistically significant differences(P<0.05).Conclusion rhG-CSF combined with PEG-rhG-CSF can maintain the balance of neutrophils in NP mice by increasing the levels of CXCR4+ in the bone marrow and CD11b+Ly6G+ in peripheral blood neutrophils.关键词
中性粒细胞减少症/重组人粒细胞集落刺激因子/聚乙二醇化重组人粒细胞集落刺激因子/细胞因子Key words
Neutropenia/Recombinant human granulocyte colony stimulating factor/Pegylated recombinant human granulocyte colony stimulating factor/Cell factor分类
医药卫生引用本文复制引用
陈适,何琳..rhG-CSF联合PEG-rhG-CSF治疗小鼠中性粒细胞减少症的研究[J].中国医药科学,2023,13(24):13-16,4.基金项目
四川省成都市卫生健康委员会科研课题(2020135). （2020135）
