中国医药科学2023，Vol.13Issue(24)：17-21,25,6.DOI:10.20116/j.issn2095-0616.2023.24.04
miR-548a-3p通过调节NLRP3炎性小体在脓毒症致ARDS中的机制研究
Study of the mechanism of miR-548a-3p in sepsis-induced ARDS by regulating NLRP3 inflammasome
摘要
Abstract
Objective In acute respiratory distress syndrome(ARDS),the mechanism of microRNA(miR)-548a-3p is explored caused by lipopolysaccharide-induced sepsis by regulating NLRP3 inflammasome.Methods A total of 45 mice were divided into a control group,an ARDS group and an ARDS+miR-548a-3p agomir group according to the random number table,with 15 mice in each group.The ARDS model was established by intranasal instillation of 6 mg/kg lipopolysaccharide into the lung,and the level of miR-548a-3p was increased by intraperitoneal injection of miR-548a-3p agomir(300 μg).HE staining,lung tissue injury score,interleukin-1β,IL-18 and NLRP3 protein levels were detected in each group.Dual-luciferase reports the targeting relationship between miR-548a-3p and NLRP3 was confirmed by using them.miR-548a-3p was overexpressed after transfection of miR-548a-3p mimic.NLRP3 as well as IL-1β and IL-18 levels,were detected by RT-qPCR and Western blot.Results The lung tissue injury score,IL-1β,IL-18 Compared with the control group and the ARDS+miR-548a-3p,the ARDS group had significantly elevated levels of NLRP3 protein expression agomir group,with statistically significant differences(P<0.05).Dual-luciferase reports indicate that miR-548a-3p can target NLRP3.The expression levels of NLRP3 mRNA and protein,IL-1β/pro-IL-1β and IL-18 in cells of the miR-548a-3p mimic group were significantly lower than those of the miR-548a-3p NC group,with statistically significant differences(P<0.05).Conclusion miR-548a-3p can inhibit the activation of NLRP3 inflammasome by targeting the inhibition of NLRP3 protein,the inflammatory injury of ARDS was mitigated by doing so.关键词
脓毒症/急性呼吸窘迫综合征/miR-548a-3p/NLRP3/炎性小体Key words
Sepsis/Severe respiratory failure syndrome/miR-548a-3p/NLRP3/Inflammasome分类
医药卫生引用本文复制引用
林文武,李玉堂,杨少东,郭春文,林增杰,朱景法..miR-548a-3p通过调节NLRP3炎性小体在脓毒症致ARDS中的机制研究[J].中国医药科学,2023,13(24):17-21,25,6.基金项目
福建省泉州市科技计划项目(2018Z141). （2018Z141）
