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Serratia sp.对铜绿微囊藻、斜生栅藻的溶藻效果OACHSSCDCSTPCD

Algae-lysing Effect of Algicidal Bacterium Serratia sp.on Microcystis aeruginosa and Scenedesmus obliquus

中文摘要英文摘要

[目的]探讨一株溶藻菌S6(Serratia sp.)对富营养化水中优势蓝藻和绿藻的溶藻效果,为有害藻类的生物控制提供参考.[方法]从富营养化水体中分离到一株溶藻菌S6(Serratia sp.,GenBank登录号为KY462187),将不同浓度菌液分别加至铜绿微囊藻(Microcystis aeruginosa)、斜生栅藻(Scenedesmus obliquus)以及两者以浓度比1∶1混合的藻液(下称"混合藻")中,进行溶藻实验,测定14d内实验体系中藻的生物量、叶绿素a、氨氮和总磷(TP)浓度,分析溶藻菌S6对不同藻类的溶藻效果以及对水体中氮磷的影响;运用光谱技术分析溶藻产物特性.[结果与结论]溶藻菌S6对铜绿微囊藻、斜生栅藻和混合藻的溶藻效果较佳,且菌液浓度越高,溶藻效果越佳,其中3组原菌液(2×109 mL-1)处理组14d内的溶藻率分别为89.4%、80%、78.6%.溶藻菌S6对3种实验体系氨氮和TP浓度影响较大,原菌液处理组变化最明显,其中铜绿微囊藻藻液氨氮和TP质量浓度分别下降21.24、6.21 mg/L;斜生栅藻藻液氨氮和TP质量浓度分别下降13.19、7.54 mg/L;混合藻液中氨氮和TP质量浓度分别下降10.81、11.85 mg/L.傅里叶红外光谱、紫外光谱和三维荧光光谱分析表明,铜绿微囊藻的溶藻产物中主要含类色氨酸、类富里酸和类腐殖酸物质,斜生栅藻的溶藻产物中主要含类色氨酸和类腐殖酸物质.

[Objective]The aim of this paper is to investigate the algae-lysing effect of an algaolytic bacterium S6(Serratia sp.)on dominant cyanobacteria and green algae in eutrophic water.[Method]The algaolytic bacterium S6(Serratia sp.,accession No.KY462187)was isolated from the eutrophic water;the bacterium at different concentrations was added to the algal solutions of Microcystis aeruginosa,Scenedesmus obliquus,and a mixture of the two in the ratios of 1∶1(Hereinafter referred to as"mixed algae").The algal biomass,concentration of chlorophyll a,NH3-N and TP of the experimental systems were measured,and the algae-lysing effect on different algae and effect on nitrogen and phosphorus of the experimental systems were analyzed in a fortnight.Then,algae-lysing products were analyzed by spectral technology of these experimental systems.[Result and Conclusion]The strain S6 had a desirable algae-lysing effect on M.aeruginosa and S.obliquus under monoculture and coculture,the higher the concentrations of bacterium,the better the algae-lysing effect,dissolving M.aeruginosa,S.obliquus and the mixed algae under coculture with a removal rate of 89.4%,80%and 78.6%in the three original bacterial suspensions.Furthermore,the concentrations of NH3-N and total phosphorus(TP)of the three experimental systems existed fluctuations significantly,those of the M.aeruginosa decreased by 21.24 and 6.21 mg/L,those of the S.obliquus decreased by 13.19 and 7.54 mg/L,those of the mixed algae decreased by 10.81 and 11.85 mg/L.The algae-lysing products of the three experimental systems were analyzed by infrared spectroscopy,ultraviolet spectroscopy and three-dimensional fluorescence spectroscopy scanning,and it was found that the algae-lysing products of M.aeruginosa mainly contained tryptophan-like,fulvic acid-like and humic acid-like substances,while the algae-lysing products of S.obliquus mainly contained tryptophan-like and humic acid-like substances.

洪桂云;汪涛;朱慧;马少雄

安徽建筑大学环境与能源工程学院/水污染控制与废水资源化安徽省重点实验室,安徽 合肥 230601

环境科学

铜绿微囊藻斜生栅藻溶藻菌Serratia sp.溶藻产物光谱分析技术

Microcystis aeruginosaScenedesmus obliquusalgicidal bacteriaSerratia sp.algae-lysing productsspectral analysis techniques

《广东海洋大学学报》 2024 (001)

76-83 / 8

安徽省自然科学基金(No.1908085141ME);安徽建筑大学校级教研项目(No.2021jy27)

10.3969/j.issn.1673-9159.2024.01.009

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