浙江医学2023，Vol.45Issue(24)：2589-2594,后插1,7.DOI:10.12056/j.issn.1006-2785.2023.45.24.2023-561
双氢青蒿素对人骨髓增生异常综合征细胞凋亡的影响及机制研究
Effect of dihydroartemisinin on apoptosis of human myelodysplastic syndrome cells and its mechanism
摘要
Abstract
Objective To explore the effect of dihydroartemisinin(DHA)on apoptosis of human myelodysplastic syndrome(MDS)cells and its mechanism.Methods Human MDS SKM-1 cells were treated in vitro with DHA,or in combination of apoptosis inhibitors or with chemotherapeutic drugs.The cell viability was detected by cell counting kit-8(CCK-8)method;the cell survival status was detected by propidium iodide(PI)staining;the intracellular reactive oxygen species(ROS)level was determined by flow cytometry.Results CCK-8 assay showed that DHA inhibited the proliferation of SKM-1 cells in a concentration-dependent manner;and the effect of DHA was blocked by apoptosis inhibitors,which was also confirmed by PI staining.After SKM-1 cells were treated by DHA plus chemotherapy drugs 5-AZ or Ara-C,the cell survival rate was significantly lower than that treated by single drugs.The flow cytometry showed that with the increase of drug concentration,the apoptosis rate and ROS levels increased.After SKM-1 cells were treated by DHA plus5-AZ or Ara-C,the ROS levels and apoptosis rate were more markedly.Conclusion DHA can induce apoptosis of SKM-1 cells in a concentration-dependent manner,generate ROS,and have a synergistic effect with 5-AZ and Ara-C.关键词
骨髓增生异常综合征/双氢青蒿素/活性氧/凋亡Key words
Myelodysplastic syndromes/Dihydroartemisinin/Reactive oxygen species/Apoptosis引用本文复制引用
周俊宇,杨蕾,张寅昊,余灵艳,阳馨仪,杜璟,童向民..双氢青蒿素对人骨髓增生异常综合征细胞凋亡的影响及机制研究[J].浙江医学,2023,45(24):2589-2594,后插1,7.基金项目
浙江省自然科学基金资助项目(BY23H080022) （BY23H080022）
