浙江医学2023，Vol.45Issue(24)：2612-2614,2621,后插5,5.DOI:10.12056/j.issn.1006-2785.2023.45.24.2023-2047
阳离子聚合物携带HIF-1α基因传递对小鼠后肢缺血的影响
PC mediated HIF-1α gene delivery for treatment of hind limb ischemia in mouse model
摘要
Abstract
Objective To investigate the effect of hypoxia-inducible factors 1 alpha(HIF-1α)gene delivered by cationic polymer(PC)for treatment of hind limb ischemia in mice.Methods The PC was electrostatically bound to HIF-1αto form PC-HIF-1α.The hind limb ischemia model was established in 18 C57BL/6 mice and the blood flow of the model mice was examined by laser Doppler at d1 and d7 after modeling.The model mice were randomly divided into PBS,PC and PC-HIF-1α groups with 6 mice in each group,100 μL PBS,PC solution or PC-HIF-1α were injected at d1,d3 and d5 after modeling,respectively.After 7 d,the ischemic gastrocnemius muscle was taken for CD31 immunofluorescence staining.The size and the intracellular trafficking of PC-HIF-1α were determined to evaluate the blood perfusion of the ischemic limb in the 3 groups of mice.Results The PC-HIF-1α had an average size of 150-200 nm.The PC-HIF-1α efficiently mediated cellular uptake and trafficking,which was dependent on cytoskeleton.The recovering of blood perfusion in the hind limb of PC-HIF-1α group was significantly higher than that of the PBS group and PC group at d7(86.00％vs.50.17％and 86.00％vs.51.00％,respectively;both P＜0.01).Intramuscular injection of PC-HIF-1α significantly increased the number of vessels/per field and the expansion of fibroblasts(both P＜0.01).Conclusion PC-HIF-1α can stimulate the early establishment of effective collateral circulation in mice with hind limb ischemia.关键词
阳离子聚合物/缺氧诱导因子1α/后肢缺血模型/血管再生Key words
Cationic polymer/Hypoxia-inducible factors 1 alpha/Hindlimb ischemia/Angiogenesis引用本文复制引用
葛云芬,毛慧,钱少杰,王静..阳离子聚合物携带HIF-1α基因传递对小鼠后肢缺血的影响[J].浙江医学,2023,45(24):2612-2614,2621,后插5,5.基金项目
浙江省公益技术研究计划/社会发展项目(LGF19H020010) （LGF19H020010）
浙江省医药卫生科技计划项目(2021KY476) （2021KY476）
