植物营养与肥料学报2023，Vol.29Issue(11)：2120-2131,12.DOI:10.11674/zwyf.2023114
杜梨响应低钾和盐胁迫基因PbHAK17的克隆及功能分析
Cloning and functional analysis of PbHAK17 from Pyrus betulifolia in response to low potassium and salt stress
摘要
Abstract
[Objectives]Pyrus betulifolia is a commonly cultivated rootstock with high salt tolerance.A potassium transporter gene,PbHAK1 7 that was screened out from Pyrus betulaefolia,seems respond to both K and salt stress.We studied its expression and function characteristics through bioinformatics,gene expression,and genetic transformation analysis.[Methods]Firstly,the PbHAK1 7 gene was cloned,and the protein structure and phylogenetic relationship were analyzed.Subcellular localization,K-deficient yeast,and transgenic Arabidopsis thaliana experiments were then employed to determine the function of the gene.The growth status,K+and Na+contents of overexpressed Arabidopsis lines and wild-type Arabidopsis lines were analyzed under K deficiency and normal K level(0 and 3 mmol/L K+),and normal condition and salt stress(50 and 200 mmol/L Na+).[Results]The PbHAK17 is 2370 bp in length and encodes 789 amino acids,and the PbHAK17 protein was localized on the cell membrane.K deficiency and salt stress induced a significant up-expression of PbHAK1 7 in the root of Pyrus betulifoliaand.In conditions of potassium deficiency,the PbHAK1 7 yeast transformant showed significant promotion effect on the growth of deficient yeast through supplementing K,and restored the growth of deficient yeast under salt stress.The K+content in leaves and roots of Arabidopsis overexpressing PbHAK1 7 was higher than that of wild-type Arabidopsis under low K treatment.Under the same Na+concentration,PbHAK1 7 transgenic Arabidopsis had larger and denser leaves than wild-type Arabidopsis,and the K+/Na+ratio of roots was significantly higher than that of wild-type Arabidopsis.[Conclusions]The pear potassium transporter gene PbHAK1 7 was localized in the plasma membrane of the cell,K deficiency and salt stress could induce the overexpression of PbHAK1 7 in the root system,increase K+accumulation in root and leaves,and regulate the K+/Na+ratio in plant,bring about stronger tolerance of Pyrus betulifolia to K deficiency and salt stress.关键词
杜梨/钾胁迫/盐胁迫/PbHAK17/酵母/功能验证Key words
Pyrus betulifolia/potassium stress/salt stress/PbHAK17/yeast/functional verification引用本文复制引用
金雨濛,杨晗,申长卫,徐阳春,董彩霞..杜梨响应低钾和盐胁迫基因PbHAK17的克隆及功能分析[J].植物营养与肥料学报,2023,29(11):2120-2131,12.基金项目
国家自然科学基金项目(32272809) （32272809）
河南省青年科学基金项目(222300420160) （222300420160）
河南科技学院2017年高层次人才引进启动项目(2017034) （2017034）
国家现代农业产业技术体系建设专项资金项目(CARS-28). （CARS-28）
