ADCY6通过上皮-间质转化抑制结直肠癌细胞增殖、迁移和侵袭OACSTPCD

Objective:To investigate the mechanism of adenylyl cyclase isoform 6(ADCY6)in suppressing CRC cell prolifera-tion,migration and invasion.Methods:The expression of ADCY6 in various tumors was investigated by bioinformatics database analy-sis.Further analysis of the association among ADCY6 expression levels and poor prognosis and the clinicopathological features of color-ectal cancer patients was performed.The expression of ADCY6 mRNA and protein in CRC cells was measured by conducting quantita-tive real-time reverse-transcription(RT-qPCR)assays and western blotting(WB).A CCK-8 assay was used to detect the effect of AD-CY6 on the proliferation of CRC cells.Transwell was used for cell migration and invasion assays.Xenograft tumours were subcutaneously injection of CRC cells transfected with the ADCY6 overexpression vector into the BALB/c mice,the animal model transplantation tumor in nude mice was constructed,tumor volume and mass was calculated and growth curve were drawed.In addition CRC cells were trans-fected with ADCY6 overexpression vector and the expression of key EMT proteins were tested to confirm ADCY6 play its role by inhibi-ting EMT.Results:The results showed that the expression of ADCY6 was dysregulated in various malignant tumors,and ADCY6 mRNA expression was lower in CRC.Additionally,ADCY6 had lower protein expression in colon cancer tissues.ADCY6 expression was correla-ted with age,tumor stage and lymph node metastasis.ADCY6 expression levels in FHC cells were higher than those in CRC cells.Over-expression of ADCY6 inhibited the proliferation,migration and invasion of CRC cells,and overexpression of ADCY6 hindered tumor growth,includes tumor volume and weight in vitro.Furthermore,upregulation of ADCY6 resulted in a dramatic decrease in the expres-sion of N-cadherin,vimentin and Ki-67 while contributing to a statistically significant increase in the expression of E-cadherin.Conclu-sion:The low expression of ADCY6 is associated with the poor prognosis of CRC patients,and ADCY6 inhibits the malignant biological behaviors of CRC cells by mediating EMT.