川北医学院学报2024，Vol.39Issue(1)：8-11,17,5.DOI:10.3969/j.issn.1005-3697.2024.01.002
柴胡皂苷D调控miR-34a对胶质瘤细胞C6增殖与自噬的影响
Effect of regulation of miR-34a by saikosaponin D on the proliferation and autophagy of glioma cell line C6
摘要
Abstract
Objective:To investigate the effect of saikosaponin D regulating miR-34a on the proliferation and autophagy of glio-ma cell line C6.Methods:Take the final concentration as 0,3,6,9,12 and 15 μmol/L after treating glioma cell C6 with saikosaponin D,the cell proliferation ability was detected by CCK-8,and the autophagy related proteins Beclin1,LC3-Ⅱ/LC3-Ⅰ,and gene expression in glioma cell C6 were detected by Western blot and qRT PCR.Divide glioma cell C6 into four groups:control group(Control group),saikosaponin D group(Ssd group),saikosaponin D +miR-34a negative control transfection group(Ssd +miR-34a NC group),saikosa-ponin D +miR-34a inhibitor transfection group(Ssd +miR-34a inhibitor group),and detected the effects of miR-34a NC and miR-34a inhibitor on the proliferation ability of glioma cell C6 after treatment with saikosaponin D,Western blot was used to detect the protein expression of autophagy related proteins LC3-Ⅰ,LC3-Ⅱ,and Beclin-1 in C6 glioma cells of each group.Results:After treatment with different concentrations of saikosaponin D for 48 h,the proliferation ability of glioma cells C6 was decreased,the ratio of autophagy-re-lated protein LC3Ⅱ/LC3-Ⅰ was increased,and the expression level of Beclin1 was increased.There was no statistically significant differ-ence in C6 proliferation rate,LC3 Ⅱ/LC3-Ⅰ ratio,and Beclin1 expression level between the Ssd group and the Ssd +miR-34a NC group(P>0.05).The proliferation rate of C6 glioma cells in the Ssd group and Ssd +miR-34a NC group was lower than that in the Control group and Ssd +miR-34a inhibitor group.The LC3 Ⅱ/LC3-Ⅰ ratio and Beclin1 expression level were higher than those in the Control group and Ssd +miR-34a inhibitor group,and the differences were statistically significant(P<0.05).The proliferation rate of C6 glio-ma cells in the Ssd +miR-34a inhibitor group was lower than that in the Control group,and the LC3 Ⅱ/LC3-Ⅰ ratio was higher than those in the Control group,with statistical significance(P<0.05).Conclusion:Chaihu saponin D may inhibit the proliferation of glio-ma cell C6 and induce autophagy by regulating miR-34a.关键词
胶质瘤/柴胡皂苷D/miR-34a/增殖/自噬Key words
Glioma/Chaihu saponin D/MiR-34a/Proliferation/Autophagy分类
医药卫生引用本文复制引用
刘晓熙,赵鹏伟,张静,于慧玲..柴胡皂苷D调控miR-34a对胶质瘤细胞C6增殖与自噬的影响[J].川北医学院学报,2024,39(1):8-11,17,5.基金项目
内蒙古自治区自然科学基金项目(2019MS08153) （2019MS08153）
