川北医学院学报2024，Vol.39Issue(1)：12-17,6.DOI:10.3969/j.issn.1005-3697.2024.01.003
DNMT1在口腔鳞状细胞癌中的表达及作用机制研究
Expression and mechanism of DNMT1 in oral squamous cell carcinoma
摘要
Abstract
Objective:To investigate the expression of DNMT1 in oral squamous cell carcinoma,and to explore its effect on cell proliferation,migration,and invasion.Methods:The GEPIA2 database was used to analyze the expression of DNMT1 in pan-cancers.RT-qPCR and Western Blot were used to detect the expression of DNMT1 in oral epithelial cell lines HOK,HSC3 and SAS.The shRNA lentivirus was used to knock down DNMT1 in HSC3 and SAS cells,and RT-qPCR and Western Blot were used to detect the knockdown efficiency of sh DNMT1.The effects of DNMT1 knockdown on the proliferation of HSC3 and SAS cells were detected by CCK-8 and plate cloning assay,and the effects of DNMT1 knockdown on the migration and invasion of HSC3 and SAS cells were detected by wound healing and Transwell assay.Results:DNMT1 mRNA and protein expressions in HSC3 and SAS cells were higher than in HOK cells(P<0.05).After DNMT1 knockdown,the proliferation,migration and invasion of HSC3 and SAS cells were decreased(P<0.05).Conclusion:DNMT1 is highly expressed in oral squamous cell carcinoma cells and can promote the proliferation,migration and invasion of oral squamous cell carcinoma cells.关键词
口腔鳞状细胞癌/DNMT1/增殖/迁移/侵袭Key words
Oral squamous cell carcinoma/DNMT1/Proliferation/Migration/Invasion分类
医药卫生引用本文复制引用
邹浩冬,杨飞,张莞嫣,王拓,邱亚,吴艳..DNMT1在口腔鳞状细胞癌中的表达及作用机制研究[J].川北医学院学报,2024,39(1):12-17,6.基金项目
川北医学院附属医院科研发展计划项目(2023JC048) （2023JC048）
川北医学院国际教育交流学院 2023 年度来华留学生教学工程建设项目(ISJG2023-14) （ISJG2023-14）
川北医学院校级科研发展项目(CBY22-QNA65) （CBY22-QNA65）
