结核分枝杆菌分泌蛋白ESAT-6、CFP-10在肺结核组织中的表达OACSTPCD
Expression of Mycobacterium tuberculosis secreted antigen ESAT-6 and CFP-10 in pulmonary tuberculosis tissue
目的:探究结核分枝杆菌特异性抗原ESAT-6、CFP-10 在肺结核组织中的表达.方法:收集经病理学诊断的 19例石蜡包埋肺结核组织标本,以20 例正常肺组织(肺癌切除标本正常组织断端)为阴性对照,采用免疫组化检测ESAT-6 及CFP-10,并与抗酸染色进行对比.结果:ESAT-6 及CFP-10 的阳性信号主要分布在结核病灶的坏死区域及其周围的巨噬细胞和多核巨细胞中,其分布与抗酸杆菌有关,范围更广.免疫组化检测ESAT-6 的敏感度、特异度分别为73.68%、90.00%;CFP-10 的敏感度和特异度分别为63.16%和95.00%;抗酸染色敏感度为 52.63%,免疫组化的敏感度高于抗酸染色(P<0.05).结论:采用免疫组化检测ESAT-6 及CFP-10 在结核诊断中有潜在的应用价值.
Objective:To detect the expression of Mycobacterium tuberculosis specific antigen ESAT-6 and CFP-10 in pulmona-ry tuberculosis tissue.Methods:A total of 19 paraffin-embedded pulmonary tuberculosis tissue specimens were collected and pathologi-cally diagnosed,and 20 cases of normal lung tissue(normal tissue stumps of lung cancer resection specimens)were negative controls,ESAT-6 and CFP-10 were detected by immunohistochemistry,and compared with acid-fast staining.Results:The positive signals of ES-AT-6 and CFP-10 were mainly distributed in the necrotic area of tuberculosis lesions and the surrounding macrophages and multinuclea-ted giant cells,and their distribution was related to acid-fast bacilli and had a wider range.The sensitivity and specificity of immunohis-tochemical detection of ESAT-6 were 73.68%and 90.00%,respectively,the sensitivity and specificity of CFP-10 were 63.16%and 95.00%,respectively,the sensitivity of acid-fast staining was52.63%.The sensitivity of immunohistochemistry was higher than that of acid-fast staining(P<0.05).Conclusion:Immunohistochemical detection of ESAT-6 and CFP-10 has potential application value in the diagnosis of tuberculosis.
陈雪倩;方诗宇;于江;段绍琪;孙杰;刘凤君
川北医学院附属医院感染科,四川 南充 637000
临床医学
传染病CFP-10ESAT-6免疫组织化学染色法肺结核
Infectious diseaseCFP-10ESAT-6Immunohistochemical stainingPulmonary tuberculosis
《川北医学院学报》 2024 (001)
18-21 / 4
南充市市校合作战略合作项目(20SXQT0017);南充市市校科技战略合作项目(19SXHZ0055);川北医学院附属医院科研项目(2020ZX003)
评论