川北医学院学报2024，Vol.39Issue(1)：18-21,4.DOI:10.3969/j.issn.1005-3697.2024.01.004
结核分枝杆菌分泌蛋白ESAT-6、CFP-10在肺结核组织中的表达
Expression of Mycobacterium tuberculosis secreted antigen ESAT-6 and CFP-10 in pulmonary tuberculosis tissue
摘要
Abstract
Objective:To detect the expression of Mycobacterium tuberculosis specific antigen ESAT-6 and CFP-10 in pulmona-ry tuberculosis tissue.Methods:A total of 19 paraffin-embedded pulmonary tuberculosis tissue specimens were collected and pathologi-cally diagnosed,and 20 cases of normal lung tissue(normal tissue stumps of lung cancer resection specimens)were negative controls,ESAT-6 and CFP-10 were detected by immunohistochemistry,and compared with acid-fast staining.Results:The positive signals of ES-AT-6 and CFP-10 were mainly distributed in the necrotic area of tuberculosis lesions and the surrounding macrophages and multinuclea-ted giant cells,and their distribution was related to acid-fast bacilli and had a wider range.The sensitivity and specificity of immunohis-tochemical detection of ESAT-6 were 73.68%and 90.00%,respectively,the sensitivity and specificity of CFP-10 were 63.16%and 95.00%,respectively,the sensitivity of acid-fast staining was52.63%.The sensitivity of immunohistochemistry was higher than that of acid-fast staining(P<0.05).Conclusion:Immunohistochemical detection of ESAT-6 and CFP-10 has potential application value in the diagnosis of tuberculosis.关键词
传染病/CFP-10/ESAT-6/免疫组织化学染色法/肺结核Key words
Infectious disease/CFP-10/ESAT-6/Immunohistochemical staining/Pulmonary tuberculosis分类
医药卫生引用本文复制引用
陈雪倩,方诗宇,于江,段绍琪,孙杰,刘凤君..结核分枝杆菌分泌蛋白ESAT-6、CFP-10在肺结核组织中的表达[J].川北医学院学报,2024,39(1):18-21,4.基金项目
南充市市校合作战略合作项目(20SXQT0017) （20SXQT0017）
南充市市校科技战略合作项目(19SXHZ0055) （19SXHZ0055）
川北医学院附属医院科研项目(2020ZX003) （2020ZX003）
