动物医学进展2024，Vol.45Issue(1)：1-6,6.
基于VP6蛋白的牛轮状病毒抗体间接ELISA检测方法的建立与应用
Establishment and Application of Indirect ELISA Method for Detection of BRV Antibody Based on VP6 Protein
摘要
Abstract
In order to establish a method for the detection of bovine rotavirus(BRV)serum antibody,bovine rotavirus VP6 gene was cloned from the diseased material and its expression vector was constructed.The recombinant VP6 protein was expressed by prokaryotic expression method.An indirect ELISA method for detection of bovine rotavirus serum antibodies was established.The results indicated that the size of the ex-pressed recombinant VP6 protein was 40 ku,and the expression form was inclusion bodies.Western blot in-dicated that the recombinant protein had good reactivity.The resulet also indicated that the optimal work-ing conditions were coated with 4 μg/mL antigen,50-fold dilution of the first antiserum and 10000-fold di-lution of the enzyme-labeled second antibody.The critical value of negative and positive samples was 0.233.The method has good specificity and sensitivity.This study established an indirect ELISA method for the detection of BRV antibody,which can be used for clinical detection of BRV infection.关键词
牛轮状病毒/VP6蛋白/原核表达/间接酶联免疫吸附试验Key words
Bovine rotavirus/VP6 protein/prokaryotic expression/indirect enzyme-linked immunosorbent assay(indirect-ELISA)分类
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刘广阔,邹敏,吴发兴,于皓同,王凯茸,张锐铮,张琪,许信刚..基于VP6蛋白的牛轮状病毒抗体间接ELISA检测方法的建立与应用[J].动物医学进展,2024,45(1):1-6,6.基金项目
陕西省重点研发计划重点项目(2022NY-098) （2022NY-098）
