动物医学进展2024，Vol.45Issue(1)：7-11,5.
血清4型禽腺病毒胶体金免疫层析检测方法的建立
Establishment of Colloidal Gold Immunochromatagraphyic Assay Detection Method for Serotype 4 Fowl Adenovirus
摘要
Abstract
In order to establish a colloidal gold immunochromatographic method for rapid detection and di-agnosis of serotype 4 fowl adenovirus(FAdV-4),the hexon-L1 protein of FAdV-4 was expressed prokary-otically expression,polyclonal antibody was prepared by immunizing rabbits,polyclonal antibody was coa-ted on the detection(T)line,goat anti-rabbit IgG was coated on the quality control(C)line,and used col-loidal gold-labeled polyclonal antibody as a tracer to prepare a colloidal gold immunochromatographic de-tection card for the detection of FAdV-4 antigens,and its sensitivity and specificity were detected.Clinical samples were detected by it and compared with PCR detection.The results showed that the prepared colloi-dal gold test card could detect samples with FAdV-4 content of 102.0945EID50,and FAdV-8,Newcastle dis-ease virus,avian leukemia virus do not show specific bands.The coincidence rate between the detection of clinical samples and PCR detection reached 98％.Studies showed that the established colloidal gold immu-nochromatagraphyic detection method can be used for the rapid diagnosis of FAdV-4 infection.关键词
血清4型禽腺病毒/六邻体环1区/重组蛋白/胶体金检测方法Key words
Fowl adenovirus-4/hexon-L1/recombinant protein/colloidal gold detection method分类
农业科技引用本文复制引用
陈乐乐,王凯莉,刘成,杜旭升,曹胜亮,李玉保,路建彪,司振书..血清4型禽腺病毒胶体金免疫层析检测方法的建立[J].动物医学进展,2024,45(1):7-11,5.基金项目
山东省重点研发计划项目(2019GNC106082) （2019GNC106082）
山东省自然科学基金项目(ZR2020MC175) （ZR2020MC175）
山东省自然科学基金面上项目(ZR202112010377) （ZR202112010377）
