血清4型禽腺病毒胶体金免疫层析检测方法的建立OACSTPCD

In order to establish a colloidal gold immunochromatographic method for rapid detection and di-agnosis of serotype 4 fowl adenovirus(FAdV-4),the hexon-L1 protein of FAdV-4 was expressed prokary-otically expression,polyclonal antibody was prepared by immunizing rabbits,polyclonal antibody was coa-ted on the detection(T)line,goat anti-rabbit IgG was coated on the quality control(C)line,and used col-loidal gold-labeled polyclonal antibody as a tracer to prepare a colloidal gold immunochromatographic de-tection card for the detection of FAdV-4 antigens,and its sensitivity and specificity were detected.Clinical samples were detected by it and compared with PCR detection.The results showed that the prepared colloi-dal gold test card could detect samples with FAdV-4 content of 102.0945EID50,and FAdV-8,Newcastle dis-ease virus,avian leukemia virus do not show specific bands.The coincidence rate between the detection of clinical samples and PCR detection reached 98％.Studies showed that the established colloidal gold immu-nochromatagraphyic detection method can be used for the rapid diagnosis of FAdV-4 infection.