山羊伪结核棒状杆菌噬菌体的分离鉴定OACSTPCD

In order to obtain effective biological agents to control the infection of Corynebacterium pseudotu-berculosis in goats,we conducted epidemiological analysis on the bacterial strains and phages sequence in-formation by NCBI accession number,and used C.pseudotuberculosis as host strain to isolate and identify the phage from sewage of a farm in Shaanxi province by double-layer plate method.The results showed that the outbreaks of C.pseudotuberculosis were globally distributed,especially in the USA and Brazil.In addi-tion,the USA was also the main source of Corynebacteriurn phage isolates.One phage was isolated which named M2 in this study.The optimal MOI of M2 was 0.1,the incubation period was 20 min,the cleavage period was 60 min,and the average cleavage volume was about 690 PFU/cell,indicating that M2 with a strong lytic capacity of C.pseudotuberculosis.This study can provide epidemiological data reference for the prevention and control of C.pseudotuberculosis,and provide a potential new tool for clinical treatment of caseous lymphadenitis caused by this pathogen.