动物医学进展2024，Vol.45Issue(1)：12-17,6.
山羊伪结核棒状杆菌噬菌体的分离鉴定
Isolation and Identification of a Bacteriophage Active against Corynebacterium pseudotuberculosis
摘要
Abstract
In order to obtain effective biological agents to control the infection of Corynebacterium pseudotu-berculosis in goats,we conducted epidemiological analysis on the bacterial strains and phages sequence in-formation by NCBI accession number,and used C.pseudotuberculosis as host strain to isolate and identify the phage from sewage of a farm in Shaanxi province by double-layer plate method.The results showed that the outbreaks of C.pseudotuberculosis were globally distributed,especially in the USA and Brazil.In addi-tion,the USA was also the main source of Corynebacteriurn phage isolates.One phage was isolated which named M2 in this study.The optimal MOI of M2 was 0.1,the incubation period was 20 min,the cleavage period was 60 min,and the average cleavage volume was about 690 PFU/cell,indicating that M2 with a strong lytic capacity of C.pseudotuberculosis.This study can provide epidemiological data reference for the prevention and control of C.pseudotuberculosis,and provide a potential new tool for clinical treatment of caseous lymphadenitis caused by this pathogen.关键词
伪结核棒状杆菌/干酪性淋巴结炎/噬菌体/山羊Key words
C.pseudotuberculosis/Caseous lymphadenitis/bacteriophage/goat分类
农业科技引用本文复制引用
李少飞,赵慧英,刘明杰,姜悦才,李登亮,曹启航,陈凤强,高士孔,陈德坤,马文涛..山羊伪结核棒状杆菌噬菌体的分离鉴定[J].动物医学进展,2024,45(1):12-17,6.基金项目
陕西省动物疫病专项项目(K3030821111) （K3030821111）
国家级研究生创新创业项目(202110712251) （202110712251）
