动物医学进展2024，Vol.45Issue(1)：18-23,6.
肉鸡圆圈病毒3型和A型多杀性巴氏杆菌混合感染的检测与分析
Detection and Analysis of Mixed Infection of Gyrovirus Type 3 and Pasteurella multocida Type A in Broilers
摘要
Abstract
In order to find out the causes of emasiation,dyspnea and increased mortality of broilers in a chicken farm in Longyan city,Fujian province,bacterial isolation and identification,16S rDNA identifica-tion,capsule typing and drug sensitivity tests were carried out,and the PCR detection of viral nucleic acid,genetic evolution analysis were also performed.The results showed that the bacteria isolated from diseased chickens formed round,slightly protruded,smooth and creamy colonies on the blood plate.The nucleotide sequence of 16S rDNA was more than 99.9％homologous to that of Pasteurella multocida published in GenBank.The capsule of the strain was type A.The bacterium was highly sensitive to ampicillin,cefazolin,cefradine and other drugs.The VP2 gene of gyrovirus type 3(GyV3)was detected in infected chickens,which had the highest homology(99.9％)with GyV3 of GenBank(MK089248).I n summary,the disease was caused by a mixed infection of gyrovirus 3 and serotype A Pasteurella multocida.关键词
圆圈病毒3型/多杀性巴氏杆菌/混合感染/病原学分析Key words
Gyrovirus 3/Pasteurella multocida/mixed infection/etiological analysis分类
农业科技引用本文复制引用
庄丽云,戴爱玲,郑新添,雷天宇,戴婷婷,吴玲玲,朱金玲,廖悦辰,牛群,包银莉,黄翠琴..肉鸡圆圈病毒3型和A型多杀性巴氏杆菌混合感染的检测与分析[J].动物医学进展,2024,45(1):18-23,6.基金项目
龙岩学院预防兽医学与生物技术福建省高等学校重点实验室开放基金项目(2022KF03) （2022KF03）
动物源性人兽共患病防控福建省高校工程研究中心开放基金项目(2022K010) （2022K010）
