南方医科大学学报2024，Vol.44Issue(1)：9-16,8.DOI:10.12122/j.issn.1673-4254.2024.01.02
RBMX通过下调PKM2抑制膀胱癌细胞的增殖、迁移、侵袭和糖酵解
RBMX overexpression inhibits proliferation,migration,invasion and glycolysis of human bladder cancer cells by downregulating PKM2
摘要
Abstract
Objective To investigate the role of RNA-binding motif protein X-linked(RBMX)in regulating the proliferation,migration,invasion and glycolysis in human bladder cancer cells.Methods A lentivirus vectors system and RNA interference technique were used to construct bladder cancer 1376 and UC-3 cell models with RBMX overexpression and knockdown,respectively,and successful cell modeling was verified using RT-qPCR and Western blotting.Proliferation and colony forming ability of the cells were evaluated using EdU assay and colony-forming assay,and cell migration and invasion abilities were determined using Transwell experiment.The expressions of glycolysis-related proteins M1 pyruvate kinase(PKM1)and M2 pyruvate kinase(PKM2)were detected using Western blotting.The effects of RBMX overexpression and knockdown on glycolysis in the bladder cancer cells were assessed using glucose and lactic acid detection kits.Results RT-qPCR and Western blotting confirmed successful construction of 1376 and UC-3 cell models with RBMX overexpression and knockdown.RBMX overexpression significantly inhibited the proliferation,clone formation,migration and invasion of bladder cancer cells,while RBMX knockdown produced the opposite effects.Western blotting results showed that RBMX overexpression increased the expression of PKM1 and decreased the expression of PKM2,while RBMX knockdown produced the opposite effects.Glucose consumption and lactate production levels were significantly lowered in the cells with RBMX overexpression(P<0.05)but increased significantly following RBMX knockdown(P<0.05).Conclusion RBMX overexpression inhibits bladder cancer progression and lowers glycolysis level in bladder cancer cells by downregulating PKM2 expression,suggesting the potential of RBMX as a molecular target for diagnosis and treatment of bladder cancer.关键词
X连锁RNA结合基序蛋白/M2型丙酮酸激酶/膀胱癌/PKM2/糖酵解Key words
RNA-binding motif protein X-linked/M2 pyruvate kinase/bladder cancer/PKM2/glycolysis引用本文复制引用
颜秋霞,张国志,胡鸿,陈彩蓉,曾鹏,黄树强,谭翠钰,周秀琴,乔静,赵晓英,冯玲,朱振杰..RBMX通过下调PKM2抑制膀胱癌细胞的增殖、迁移、侵袭和糖酵解[J].南方医科大学学报,2024,44(1):9-16,8.基金项目
国家自然科学基金青年基金(82002671) （82002671）
广东省基础与应用基础研究基金(2019A1515010249) （2019A1515010249）
清远市科技计划项目(2020KJJH023) （2020KJJH023）
广东省中医药局科研项目(20241387) （20241387）
广州医科大学附属第六医院/清远市人民医院开放课题基金(202301-306) （202301-306）
广东省大学生创新训练计划项目(S202310570055) （S202310570055）
广州医科大学第六临床学院大学生科技创新项目(019,018) Supported by National Natural Science Foundation of China(82002671). （019,018）
