南方医科大学学报2024，Vol.44Issue(1)：17-24,8.DOI:10.12122/j.issn.1673-4254.2024.01.03
过表达LncRNA MEG3通过促进铁死亡增强肝癌细胞对顺铂的化疗敏感性
Overexpression of LncRNA MEG3 promotes ferroptosis and enhances chemotherapy sensitivity of hepatocellular carcinoma cells to cisplatin
摘要
Abstract
Objective To investigate the effect of overexpression of LncRNA MEG3 on proliferation,migration and cisplatin sensitivity of hepatoma cells HepG2 and LM3 and explore the underlying and mechanism.Methods The expression of MEG3 in healthy individuals and patients with hepatocellular carcinoma(HCC)was analyzed by online bioinformatics analysis,and Real-time fluorescence quantitative PCR(qRT-PCR)was used to detect MEG3 expression in different HCC cell lines.A MEG3-overexpresing plasmid was transfected in HepG2 and LM3 cells,and the changes in cell proliferation and migration were examined using CCK8 assay and scratch assay.CCK8 assay was used to determine the inhibitory rate of cisplatin on the transfected cells.A reactive oxygen species(ROS)fluorescence probe(DCFH-DA)and malondialdehyde(MDA)kit were used to assess the changes in ROS production and MDA level in the cells.Western blotting was performed to detect the expression levels of ferroptosis-related proteins glutathione peroxidase 4(GPX4)and ferritin heavy chain 1(FTH1).Results The expression of MEG3 was significantly lower in HCC cells than in LO2 cells,which was consistent with the results of bioinformatic analysis(P<0.05).Overexpression of MEG3 in the HCC cell lines significantly suppressed cell proliferation and migration(P<0.05),increased the cell inhibition rate of cisplatin(P<0.05),enhanced cellular ROS production and increased MDA levels in the cells(P<0.05).MEG3 overexpression significantly decreased the expressions of GPX4 and FTH1 in the HCC cell lines.Conclusion The expression of MEG3 is decreased in HCC cells,and its overexpression inhibits proliferation and migration and enhances cisplatin sensitivity of HCC cells by promoting ferroptosis of the cells.关键词
肝细胞癌/LncRNA MEG3/顺铂/化疗敏感性/铁死亡Key words
hepatocellular carcinoma/LncRNA MEG3/cisplatin/sensitivity to chemotherapy/ferroptosis引用本文复制引用
朱权,黄柏胜,位磊艳,罗奇志..过表达LncRNA MEG3通过促进铁死亡增强肝癌细胞对顺铂的化疗敏感性[J].南方医科大学学报,2024,44(1):17-24,8.基金项目
湖南省自然科学基金(2019JJ40398,2020JJ4768) （2019JJ40398,2020JJ4768）
长沙市2022年度指导性科技计划项目(kzd22002) （kzd22002）
