南方医科大学学报2024，Vol.44Issue(1)：45-51,7.DOI:10.12122/j.issn.1673-4254.2024.01.06
白藜芦醇可减轻高糖诱导的心肌细胞肥大:基于促进SIRT1表达维持线粒体稳态
Resveratrol alleviates hyperglycemia-induced cardiomyocyte hypertrophy by maintaining mitochondrial homeostasis via enhancing SIRT1 expression
摘要
Abstract
Objective To investigate whether resveratrol alleviates hyperglycemia-induced cardiomyocyte hypertrophy by enhancing the expression of silent information regulation 2 homolog 1(SIRT1)to maintain mitochondrial homeostasis.Methods Rat cardiomyocytes H9c2 cells with or without lentivirus-mediated mRNA interference of SIRT1 were cultured in high glucose(HG)and treated with resveratrol for 72 h.The changes in superoxide dismutase(SOD)activity,malondialdehyde(MDA)content,reactive oxygen species(ROS)level,and relative surface of the cells were examined,and the mRNA expressions of atrial natriuretic factor(ANF)and brain natriuretic peptide(BNP)and protein expressions of SIRT1,mitochondrial fusion related proteins optic atrophy protein 1(OPA1)and mitofusin 2,mitochondrial division related proteins dynamin-related protein 1(DRP1)and fission protein 1(FIS1),and mitophagy-related proteins BNIP3L and LC3 were detected using RT-qPCR and Western blotting.Results HG exposure significantly decreased SOD activity,increased MDA content,ROS production,relative cell surface,and the mRNA expressions of ANF and BNP in the cardiomyocytes;the protein expressions of SIRT1,OPA1,mitofusin 2 and BNIP3L and LC3-Ⅱ/LC3-Ⅰ ratio were all decreased and the protein expressions of DRP1 and FIS1 increased in HG-exposed cells(P<0.01).All these changes in HG-exposed cardiomyocytes were significantly alleviated by treatment with resveratrol(P<0.05).The protective effects of resveratrol against HG exposure in the cardiomyocytes were obviously attenuated by transfection of the cells with si-SIRT1(P<0.05).Conclusion Resveratrol inhibits hyperglycemia-induced cardiomyocyte hypertrophy by reducing oxidative stress,the mechanisms of which involve enhancement of SIRT1 protein expression,regulation of mitochondrial fusion and division balance,and promoting BNIP3L-mediated mitophagy to maintain mitochondrial homeostasis in the cells.关键词
糖尿病心肌病/心肌肥大/白藜芦醇/线粒体/氧化应激/SIRT1Key words
diabetic cardiomyopathy/cardiac hypertrophy/resveratrol/mitochondrial/oxidative stress/SIRT1引用本文复制引用
叶红伟,张钰明,云琦,杜若丽,李璐,李玉萍,高琴..白藜芦醇可减轻高糖诱导的心肌细胞肥大:基于促进SIRT1表达维持线粒体稳态[J].南方医科大学学报,2024,44(1):45-51,7.基金项目
安徽省教育厅科学研究重点项目(KJ2021A0762) （KJ2021A0762）
蚌埠医学院512 人才项目(by51201102) （by51201102）
安徽省优秀科研创新团队项目(2022AH010083) （2022AH010083）
